Any feedback?
Literature summary for 2.7.2.4 extracted from
- Characterization of aspartate kinase III of Bacillus subtilis (2001), Biosci. Biotechnol. Biochem., 65, 1391-1394.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene yclM introduced into aspartate kinase deficient Escherichia coli cells | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-lysine | - |
Bacillus subtilis |  |
| L-threonine | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21 | - |
L-aspartate | pH 7.0, 30°C | Bacillus subtilis | |
| 23.5 | - |
ATP | pH 7.0, 30°C | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
recombinant protein, expressed in E. coli, gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate | Bacillus subtilis | first step of a branched biosynthetic pathway for L-lysine, L-threonine, L-isoleucine and L-methionine, regulated by the end products through feedback inhibition, the 3 aspartate kinases I, II and II are regulated by different end products | ADP + 4-phospho-L-aspartate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, expressed in Escherichia coli Gif106M1 | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.62 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, all activity is lost in 1 day | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate | - |
Bacillus subtilis | ADP + 4-phospho-L-aspartate | - |
r | |
| ATP + L-aspartate | first step of a branched biosynthetic pathway for L-lysine, L-threonine, L-isoleucine and L-methionine, regulated by the end products through feedback inhibition, the 3 aspartate kinases I, II and II are regulated by different end products | Bacillus subtilis | ADP + 4-phospho-L-aspartate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aspartate kinase III | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.333 | - |
aspartate | pH 7.0, 30°C | Bacillus subtilis | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
very unstable in 10 mM tris-HCl buffer, stabilized by addition of 500 mM ammoinium sulfate | Bacillus subtilis |
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