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Literature summary for 2.7.2.3 extracted from

  • Cheng, S.F.; Huang, Y.P.; Chen, L.H.; Hsu, Y.H.; Tsai, C.H.
    Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants (2013), Plant Physiol., 163, 1598-1608.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of two mislocalized GFP-tagged PGK mutants NOTP-PGK and NLS-PGK in cytoplasm and nucleus, respectively Nicotiana benthamiana

Protein Variants

Protein Variants Comment Organism
additional information construction of GFP-tagged mutants: the transit peptide deletion mutant NOTP-PGK and the nucleus location mutant NLS-PGK. Generation of PGK-knockdown protoplasts Nicotiana benthamiana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast chl-PGK is encoded in the nucleus and translated to produce a 50-kD precursor protein and is then processed into mature 43 kD in the chloroplast Nicotiana benthamiana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Nicotiana benthamiana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
-
Nicotiana benthamiana
50000
-
-
Nicotiana benthamiana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-phospho-D-glycerate Nicotiana benthamiana
-
ADP + 3-phospho-D-glyceroyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana benthamiana Q42961
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification chl-PGK is encoded in the nucleus and translated to produce a 50-kD precursor protein and is then processed into mature 43 kD in the chloroplast Nicotiana benthamiana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana benthamiana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
Nicotiana benthamiana ADP + 3-phospho-D-glyceroyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 50000, premature enzyme, SDS-PAGE, x * 43000, n´mature enzyme, SDS-PAGE Nicotiana benthamiana

Synonyms

Synonyms Comment Organism
chl-PGK
-
Nicotiana benthamiana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Nicotiana benthamiana

General Information

General Information Comment Organism
malfunction reduction of PGK decreases the accumulation of Bamboo mosaic virus BaMV coat protein Nicotiana benthamiana
physiological function phosphoglycerate kinase is an ATP-generating enzyme that acts in the glycolytic, gluconeogenic, and photosynthetic pathways. Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants, the BaMV replicative intermediate exists in chloroplasts Nicotiana benthamiana