Literature summary for 2.7.2.3 extracted from

Joshi, R.; Karan, R.; Singla-Pareek, S.L.; Pareek, A.
Ectopic expression of Pokkali phosphoglycerate kinase-2 (OsPGK2-P) improves yield in tobacco plants under salinity stress (2016), Plant Cell Rep., 35, 27-41.

Cloned(Commentary)

Cloned (Comment)	Organism
gene OsPgk2a-P or Os02g07260, genotype Pokkali, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant ectopic expression of splicing variant OsPgk2a-P in transgenic tobacco by Agrobacterium-mediated transformation improves its salinity stress tolerance by higher chlorophyll retention and enhances proline accumulation, besides maintaining better ion homeostasis. Ectopically expressing OsPgk2a-P transgenic tobacco plants show a tall phenotype with more number of pods than wild-type plants. The number of stomata in wild-type and ectopically expressing OsPgk2a-P plants. Transgenic plants show significantly higher stomatal density than wild-type plants. Morphology of stomata is normal and no heteromorphic stomata are observed	Oryza sativa

Cloned (Comment)

Organism

gene OsPgk2a-P or Os02g07260, genotype Pokkali, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant ectopic expression of splicing variant OsPgk2a-P in transgenic tobacco by Agrobacterium-mediated transformation improves its salinity stress tolerance by higher chlorophyll retention and enhances proline accumulation, besides maintaining better ion homeostasis. Ectopically expressing OsPgk2a-P transgenic tobacco plants show a tall phenotype with more number of pods than wild-type plants. The number of stomata in wild-type and ectopically expressing OsPgk2a-P plants. Transgenic plants show significantly higher stomatal density than wild-type plants. Morphology of stomata is normal and no heteromorphic stomata are observed

Oryza sativa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42100	-	-	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 3-phospho-D-glycerate	Oryza sativa	-	ADP + 3-phospho-D-glyceroyl phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	Q6H6C7	salt tolerant landrace cv. Pokkali and salt-sensitive cv. IR64, gene OsPgk2a or Os02g07260, isozyme OsPGK2a	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Oryza sativa	-
additional information	very high transcript abundance for OsPgk2 is noted at all the developmental stages, especially at two most stress sensitive developmental stages, i.e. seedling and reproductive stage. High levels of OsPGK2 protein are detected in shoots of Pokkali even under non-stress conditions while the salinity-sensitive cv. IR64 does not show detectable levels even after 24 h of salinity stress	Oryza sativa	-
seed	-	Oryza sativa	-
seedling	-	Oryza sativa	-
shoot	-	Oryza sativa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 3-phospho-D-glycerate	-	Oryza sativa	ADP + 3-phospho-D-glyceroyl phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 42100, about, sequence calculation	Oryza sativa

Synonyms

Synonyms	Comment	Organism
OsPGK2	-	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Oryza sativa

pI Value

Organism	Comment	pI Value Maximum	pI Value
Oryza sativa	sequence calculation	-	5.57

Expression

Organism	Comment	Expression
Oryza sativa	expression of OsPGK2 in cv. IR64 is induced late, i.e., from 48 h of salinity stress till 96 h. In contrast, in case of landrace Pokkali, its expression is seen at a very basal level even in control samples which shows a continuous increase up to 96 h of salinity stress. High levels of OsPGK2 protein are detected in shoots of Pokkali even under non-stress conditions while the salinity-sensitive cv. IR64 does not show detectable levels even after 24 h of salinity stress	up

General Information

General Information	Comment	Organism
physiological function	phosphoglycerate kinase plays a major role for ATP production during glycolysis and 1, 3-bisphosphoglycerate production to participate in the Calvin cycle for carbon fixation in plants	Oryza sativa