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Literature summary for 2.7.2.3 extracted from

  • Hong, Y.; Huang, L.; Yang, J.; Cao, X.; Han, Q.; Zhang, M.; Han, Y.; Fu, Z.; Zhu, C.; Lu, K.; Li, X.; Lin, J.
    Cloning, expression and enzymatic characterization of 3-phosphoglycerate kinase from Schistosoma japonicum (2015), Exp. Parasitol., 159, 37-45.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pgk1, sequence comparisons and phylogenetic tree, real-time quantitative reverse transcription PCR enzyme expression analysis, recombinant expression of the his-tagged enzyme in Escherichia coli strain BL21 in supernatant and inclusion bodies Schistosoma japonicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Schistosoma japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
-
Schistosoma japonicum
47000
-
-
Schistosoma japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-phospho-D-glycerate Schistosoma japonicum
-
ADP + 3-phospho-D-glyceroyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Schistosoma japonicum C1LT16
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatgraphy Schistosoma japonicum

Source Tissue

Source Tissue Comment Organism Textmining
additional information Differential expression of SjPGK during development, overview. The enzyme SjPGK is expressed in all investigated developmental stages and at a higher transcript levels in 21- and 42-d worms. The SjPGK mRNA level is significantly downregulated in 10-d schistosomula from Wistar rats (non-susceptible host) Schistosoma japonicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
125
-
purified recombinant enzyme, pH 7.6, 25°C Schistosoma japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
Schistosoma japonicum ADP + 3-phospho-D-glyceroyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 44000, about, sequence calculation, x * 47000, recombinant His-tagged enzyme, SDS-PAGE Schistosoma japonicum

Synonyms

Synonyms Comment Organism
3-phosphoglycerate kinase
-
Schistosoma japonicum
PGK1 UniProt Schistosoma japonicum
SjPGK
-
Schistosoma japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
recombinant enzyme Schistosoma japonicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity range, profile, overview Schistosoma japonicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 recombinant enzyme, highly stable at Schistosoma japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 recombinant enzyme Schistosoma japonicum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.5 activity range, profile, overview Schistosoma japonicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 9 recombinant enzyme, highly stable at Schistosoma japonicum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Schistosoma japonicum

pI Value

Organism Comment pI Value Maximum pI Value
Schistosoma japonicum sequence calculation
-
6.57