Cloned (Comment) | Organism |
---|---|
gene pgk1, sequence comparisons and phylogenetic tree, real-time quantitative reverse transcription PCR enzyme expression analysis, recombinant expression of the his-tagged enzyme in Escherichia coli strain BL21 in supernatant and inclusion bodies | Schistosoma japonicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Schistosoma japonicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
- |
Schistosoma japonicum |
47000 | - |
- |
Schistosoma japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | Schistosoma japonicum | - |
ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma japonicum | C1LT16 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatgraphy | Schistosoma japonicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Differential expression of SjPGK during development, overview. The enzyme SjPGK is expressed in all investigated developmental stages and at a higher transcript levels in 21- and 42-d worms. The SjPGK mRNA level is significantly downregulated in 10-d schistosomula from Wistar rats (non-susceptible host) | Schistosoma japonicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
125 | - |
purified recombinant enzyme, pH 7.6, 25°C | Schistosoma japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Schistosoma japonicum | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, about, sequence calculation, x * 47000, recombinant His-tagged enzyme, SDS-PAGE | Schistosoma japonicum |
Synonyms | Comment | Organism |
---|---|---|
3-phosphoglycerate kinase | - |
Schistosoma japonicum |
PGK1 | UniProt | Schistosoma japonicum |
SjPGK | - |
Schistosoma japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant enzyme | Schistosoma japonicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | activity range, profile, overview | Schistosoma japonicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | recombinant enzyme, highly stable at | Schistosoma japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | recombinant enzyme | Schistosoma japonicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | activity range, profile, overview | Schistosoma japonicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | recombinant enzyme, highly stable at | Schistosoma japonicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Schistosoma japonicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Schistosoma japonicum | sequence calculation | - |
6.57 |