Crystallization (Comment) | Organism |
---|---|
crystallization via precipitation with ammonium sulfate stepwise from 60% to 75% w/v at room temperature for 2 h, then 4°C, several days | Homo sapiens |
General Stability | Organism |
---|---|
crystalline enzyme is rather unstable, dried crystals or dilute aqueous solutions lose all activity within a few weeks | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | partial inhibition in presence of Mg2+ | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace Mg2+ in activation | Homo sapiens | |
Ca2+ | 30% of the activity with Mg2+ | Homo sapiens | |
divalent cation | requires Mg2+ or Ca2+ | Homo sapiens | |
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
580 | - |
purified crystalline enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, crystals as a suspension in 3 M (NH4)2SO4, 1 mM sodium diphosphate, 1 mM EDTA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | absolute specificity for 3-phospho-D-glycerate | Homo sapiens | ADP + 1,3-diphosphoglycerate | - |
r | |
GTP + 3-phospho-D-glycerate | 60% of the activity with ATP | Homo sapiens | GDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | 64% of the activity with ATP | Homo sapiens | IDP + 1,3-diphosphoglycerate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens | |
ATP | required as phosphate donor | Homo sapiens | |
GTP | - |
Homo sapiens | |
ITP | - |
Homo sapiens |