Any feedback?
Literature summary for 2.7.2.3 extracted from
- Human phosphoglycerate kinase. I. Crystallization and characterization of normal enzyme (1972), J. Biol. Chem., 247, 440-445.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization via precipitation with ammonium sulfate stepwise from 30-35% to 65% w/v within 24 h | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| dissolved cyrstallized enzyme at low concentration is rapidly inactivated at neutral pH | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | phosphate buffer | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cation | Mg2+ or Mn2+ | Homo sapiens | |
| Mg2+ | 5 mM Mg2+ or 10 mM Mn2+ required | Homo sapiens | |
| Mn2+ | Mn2+ or Mg2+ required | Homo sapiens | |
| Mn2+ | 5 mM Mg2+ or 10 mM Mn2+ required | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49600 | - |
sedimentation equilibrium method | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 650 | 700 | purified enzyme, at 25°C | Homo sapiens |
| 1000 | - |
purified enzyme, at 35°C | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, with 10 mg/ml bovine serum albumin, stable for at least 1 week | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Homo sapiens | ADP + 1,3-diphosphoglycerate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens | |
| ATP | required as phosphate donor | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
