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Literature summary for 2.7.2.3 extracted from
- Purification of 3-phosphoglycerate kinase from diverse sources by affinity elution chromatography (1978), Biochem. J., 175, 311-319.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Gallus gallus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Beta vulgaris | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Rattus norvegicus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Sus scrofa | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Saccharomyces cerevisiae | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Bos taurus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Oryctolagus cuniculus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Ovis aries | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Spinacia oleracea | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Equus caballus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Cyprinus carpio | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Oncorhynchus mykiss | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Columba sp. | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Salmo trutta | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Trichosurus vulpecula | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Macropus giganteus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Macropus fuliginosus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Thylogale billardierii | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Pseudocheirus peregrinus | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Bufo vulgaris | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Dromaius novaehollandiae | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Abramis brama | |
| additional information | - |
additional information | Km-value for MgATP, activation energy | Chrysophrys guttulatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | 48000 | gel filtration | Gallus gallus |
| 45000 | 48000 | gel filtration | Beta vulgaris |
| 45000 | 48000 | gel filtration | Rattus norvegicus |
| 45000 | 48000 | gel filtration | Sus scrofa |
| 45000 | 48000 | gel filtration | Saccharomyces cerevisiae |
| 45000 | 48000 | gel filtration | Bos taurus |
| 45000 | 48000 | gel filtration | Oryctolagus cuniculus |
| 45000 | 48000 | gel filtration | Spinacia oleracea |
| 45000 | 48000 | gel filtration | Equus caballus |
| 45000 | 48000 | gel filtration | Cyprinus carpio |
| 45000 | 48000 | gel filtration | Oncorhynchus mykiss |
| 45000 | 48000 | gel filtration | Columba sp. |
| 45000 | 48000 | gel filtration | Salmo trutta |
| 45000 | 48000 | gel filtration | Trichosurus vulpecula |
| 45000 | 48000 | gel filtration | Macropus giganteus |
| 45000 | 48000 | gel filtration | Macropus fuliginosus |
| 45000 | 48000 | gel filtration | Thylogale billardierii |
| 45000 | 48000 | gel filtration | Pseudocheirus peregrinus |
| 45000 | 48000 | gel filtration | Bufo vulgaris |
| 45000 | 48000 | gel filtration | Dromaius novaehollandiae |
| 45000 | 48000 | gel filtration | Abramis brama |
| 45000 | 48000 | gel filtration | Chrysophrys guttulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Abramis brama | - |
bream | - |
| Beta vulgaris | - |
silver beet | - |
| Bos taurus | - |
ox | - |
| Bufo vulgaris | - |
toad | - |
| Chrysophrys guttulatus | - |
red snapper | - |
| Columba sp. | - |
- |
- |
| Cyprinus carpio | - |
carp | - |
| Dromaius novaehollandiae | - |
emu | - |
| Equus caballus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Macropus fuliginosus | - |
Western grey kangaroo | - |
| Macropus giganteus | - |
Eastern grey kangaroo | - |
| Oncorhynchus mykiss | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Pseudocheirus peregrinus | - |
ringtail possum | - |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Salmo trutta | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
| Sus scrofa | - |
- |
- |
| Thylogale billardierii | - |
- |
- |
| Trichosurus vulpecula | - |
brushtail possum | - |
| Trichosurus vulpecula | - |
vulepeculus | - |
| Vombatus ursinus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
- |
Beta vulgaris |
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
- |
Ovis aries |
- |
Spinacia oleracea |
- |
Equus caballus |
- |
Columba sp. |
- |
Salmo trutta |
- |
Trichosurus vulpecula |
- |
Vombatus ursinus |
- |
Macropus giganteus |
- |
Macropus fuliginosus |
- |
Thylogale billardierii |
- |
Pseudocheirus peregrinus |
- |
Bufo vulgaris |
- |
Dromaius novaehollandiae |
- |
Abramis brama |
- |
Chrysophrys guttulatus |
| from liver and muscle | Bos taurus |
| from muscle and liver | Rattus norvegicus |
| from muscle and liver | Oryctolagus cuniculus |
| purification from diverse sources by affinity elution chromatography | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Beta vulgaris | - |
| leaf | - |
Spinacia oleracea | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| muscle | - |
Gallus gallus | - |
| muscle | - |
Rattus norvegicus | - |
| muscle | - |
Sus scrofa | - |
| muscle | - |
Bos taurus | - |
| muscle | - |
Oryctolagus cuniculus | - |
| muscle | - |
Ovis aries | - |
| muscle | - |
Equus caballus | - |
| muscle | - |
Cyprinus carpio | - |
| muscle | - |
Oncorhynchus mykiss | - |
| muscle | - |
Columba sp. | - |
| muscle | - |
Salmo trutta | - |
| muscle | - |
Trichosurus vulpecula | - |
| muscle | - |
Vombatus ursinus | - |
| muscle | - |
Macropus giganteus | - |
| muscle | - |
Macropus fuliginosus | - |
| muscle | - |
Thylogale billardierii | - |
| muscle | - |
Pseudocheirus peregrinus | - |
| muscle | - |
Bufo vulgaris | - |
| muscle | - |
Dromaius novaehollandiae | - |
| muscle | - |
Abramis brama | - |
| muscle | - |
Chrysophrys guttulatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 300 | - |
purified liver enzyme | Rattus norvegicus |
| 460 | - |
purified muscle enzyme | Rattus norvegicus |
| 470 | - |
purified enzyme | Bufo vulgaris |
| 480 | - |
purified enzyme | Escherichia coli |
| 540 | - |
purified liver enzyme | Oryctolagus cuniculus |
| 560 | - |
purified enzyme | Abramis brama |
| 600 | - |
purified enzyme | Macropus giganteus |
| 620 | - |
purified enzyme | Columba sp. |
| 650 | - |
purified enzyme | Macropus fuliginosus |
| 650 | - |
purified liver enzyme | Bos taurus |
| 680 | - |
purified enzyme | Gallus gallus |
| 680 | - |
purified enzyme | Ovis aries |
| 690 | - |
purified enzyme | Salmo trutta |
| 690 | - |
purified enzyme | Pseudocheirus peregrinus |
| 690 | - |
purified enzyme | Chrysophrys guttulatus |
| 700 | - |
- |
Ovis aries |
| 700 | - |
purified enzyme | Sus scrofa |
| 700 | - |
purified muscle enzyme | Saccharomyces cerevisiae |
| 700 | - |
purified muscle enzyme | Oryctolagus cuniculus |
| 700 | - |
purified muscle enzyme | Equus caballus |
| 700 | - |
purified muscle enzyme | Trichosurus vulpecula |
| 700 | - |
purified muscle enzyme | Vombatus ursinus |
| 710 | - |
purified muscle enzyme | Bos taurus |
| 720 | - |
- |
Ovis aries |
| 720 | - |
purified enzyme | Oncorhynchus mykiss |
| 720 | - |
purified enzyme | Dromaius novaehollandiae |
| 780 | - |
purified enzyme | Beta vulgaris |
| 780 | - |
purified enzyme | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Gallus gallus | ADP + 1,3-diphosphoglycerate | - |
r |  |
| ATP + 3-phospho-D-glycerate | - |
Beta vulgaris | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Escherichia coli | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Rattus norvegicus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Sus scrofa | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Saccharomyces cerevisiae | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Bos taurus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Oryctolagus cuniculus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Ovis aries | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Spinacia oleracea | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Equus caballus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Cyprinus carpio | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Oncorhynchus mykiss | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Columba sp. | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Salmo trutta | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Trichosurus vulpecula | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Vombatus ursinus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Macropus giganteus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Macropus fuliginosus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Thylogale billardierii | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Pseudocheirus peregrinus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Bufo vulgaris | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Dromaius novaehollandiae | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Abramis brama | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Chrysophrys guttulatus | ADP + 1,3-diphosphoglycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Ovis aries |
| monomer | 1 * 45000-48000, SDS-PAGE | Gallus gallus |
| monomer | 1 * 45000-48000, SDS-PAGE | Beta vulgaris |
| monomer | 1 * 45000-48000, SDS-PAGE | Rattus norvegicus |
| monomer | 1 * 45000-48000, SDS-PAGE | Sus scrofa |
| monomer | 1 * 45000-48000, SDS-PAGE | Saccharomyces cerevisiae |
| monomer | 1 * 45000-48000, SDS-PAGE | Bos taurus |
| monomer | 1 * 45000-48000, SDS-PAGE | Oryctolagus cuniculus |
| monomer | 1 * 45000-48000, SDS-PAGE | Spinacia oleracea |
| monomer | 1 * 45000-48000, SDS-PAGE | Equus caballus |
| monomer | 1 * 45000-48000, SDS-PAGE | Cyprinus carpio |
| monomer | 1 * 45000-48000, SDS-PAGE | Oncorhynchus mykiss |
| monomer | 1 * 45000-48000, SDS-PAGE | Columba sp. |
| monomer | 1 * 45000-48000, SDS-PAGE | Salmo trutta |
| monomer | 1 * 45000-48000, SDS-PAGE | Trichosurus vulpecula |
| monomer | 1 * 45000-48000, SDS-PAGE | Macropus giganteus |
| monomer | 1 * 45000-48000, SDS-PAGE | Macropus fuliginosus |
| monomer | 1 * 45000-48000, SDS-PAGE | Thylogale billardierii |
| monomer | 1 * 45000-48000, SDS-PAGE | Pseudocheirus peregrinus |
| monomer | 1 * 45000-48000, SDS-PAGE | Bufo vulgaris |
| monomer | 1 * 45000-48000, SDS-PAGE | Dromaius novaehollandiae |
| monomer | 1 * 45000-48000, SDS-PAGE | Abramis brama |
| monomer | 1 * 45000-48000, SDS-PAGE | Chrysophrys guttulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Gallus gallus | |
| ADP | - |
Beta vulgaris | |
| ADP | - |
Escherichia coli | |
| ADP | - |
Rattus norvegicus | |
| ADP | - |
Sus scrofa | |
| ADP | - |
Saccharomyces cerevisiae | |
| ADP | - |
Bos taurus | |
| ADP | - |
Oryctolagus cuniculus | |
| ADP | - |
Ovis aries | |
| ADP | - |
Spinacia oleracea | |
| ADP | - |
Equus caballus | |
| ADP | - |
Cyprinus carpio | |
| ADP | - |
Oncorhynchus mykiss | |
| ADP | - |
Columba sp. | |
| ADP | - |
Salmo trutta | |
| ADP | - |
Trichosurus vulpecula | |
| ADP | - |
Vombatus ursinus | |
| ADP | - |
Macropus giganteus | |
| ADP | - |
Macropus fuliginosus | |
| ADP | - |
Thylogale billardierii | |
| ADP | - |
Pseudocheirus peregrinus | |
| ADP | - |
Bufo vulgaris | |
| ADP | - |
Dromaius novaehollandiae | |
| ADP | - |
Abramis brama | |
| ADP | - |
Chrysophrys guttulatus | |
| ATP | true substrate is the magnesium complexes of ATP | Gallus gallus | |
| ATP | true substrate is the magnesium complexes of ATP | Beta vulgaris | |
| ATP | true substrate is the magnesium complexes of ATP | Escherichia coli | |
| ATP | true substrate is the magnesium complexes of ATP | Sus scrofa | |
| ATP | true substrate is the magnesium complexes of ATP | Saccharomyces cerevisiae | |
| ATP | true substrate is the magnesium complexes of ATP | Bos taurus | |
| ATP | true substrate is the magnesium complexes of ATP | Oryctolagus cuniculus | |
| ATP | true substrate is the magnesium complexes of ATP | Ovis aries | |
| ATP | true substrate is the magnesium complexes of ATP | Spinacia oleracea | |
| ATP | true substrate is the magnesium complexes of ATP | Equus caballus | |
| ATP | true substrate is the magnesium complexes of ATP | Cyprinus carpio | |
| ATP | true substrate is the magnesium complexes of ATP | Oncorhynchus mykiss | |
| ATP | true substrate is the magnesium complexes of ATP | Columba sp. | |
| ATP | true substrate is the magnesium complexes of ATP | Salmo trutta | |
| ATP | true substrate is the magnesium complexes of ATP | Trichosurus vulpecula | |
| ATP | true substrate is the magnesium complexes of ATP | Macropus giganteus | |
| ATP | true substrate is the magnesium complexes of ATP | Macropus fuliginosus | |
| ATP | true substrate is the magnesium complexes of ATP | Thylogale billardierii | |
| ATP | true substrate is the magnesium complexes of ATP | Pseudocheirus peregrinus | |
| ATP | true substrate is the magnesium complexes of ATP | Bufo vulgaris | |
| ATP | true substrate is the magnesium complexes of ATP | Dromaius novaehollandiae | |
| ATP | true substrate is the magnesium complexes of ATP | Abramis brama | |
| ATP | true substrate is the magnesium complexes of ATP | Chrysophrys guttulatus | |
| ATP | required as phosphate donor | Gallus gallus | |
| ATP | required as phosphate donor | Beta vulgaris | |
| ATP | required as phosphate donor | Escherichia coli | |
| ATP | required as phosphate donor | Rattus norvegicus | |
| ATP | required as phosphate donor | Sus scrofa | |
| ATP | required as phosphate donor | Saccharomyces cerevisiae | |
| ATP | required as phosphate donor | Bos taurus | |
| ATP | required as phosphate donor | Oryctolagus cuniculus | |
| ATP | required as phosphate donor | Ovis aries | |
| ATP | required as phosphate donor | Spinacia oleracea | |
| ATP | required as phosphate donor | Equus caballus | |
| ATP | required as phosphate donor | Cyprinus carpio | |
| ATP | required as phosphate donor | Oncorhynchus mykiss | |
| ATP | required as phosphate donor | Columba sp. | |
| ATP | required as phosphate donor | Salmo trutta | |
| ATP | required as phosphate donor | Trichosurus vulpecula | |
| ATP | required as phosphate donor | Vombatus ursinus | |
| ATP | required as phosphate donor | Macropus giganteus | |
| ATP | required as phosphate donor | Macropus fuliginosus | |
| ATP | required as phosphate donor | Thylogale billardierii | |
| ATP | required as phosphate donor | Pseudocheirus peregrinus | |
| ATP | required as phosphate donor | Bufo vulgaris | |
| ATP | required as phosphate donor | Dromaius novaehollandiae | |
| ATP | required as phosphate donor | Abramis brama | |
| ATP | required as phosphate donor | Chrysophrys guttulatus | |
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