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Literature summary for 2.7.2.3 extracted from

  • Scopes, R.K.
    3-Phosphoglycerate kinase of baker's yeast (1975), Methods Enzymol., 42C, 134-138.
No PubMed abstract available
Search for more literature for 2.7.2.3

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization from ammonium sulfate precipitation, pH 7.0, room temperature, a few days, X-ray structure determination, in presence of 1% 1,4-dioxane and 68% ammonium sulfate, and analysis Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ATP4- weak Saccharomyces cerevisiae
additional information no inhibition by Hg2+ Saccharomyces cerevisiae
Zn2+ strong Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae
Cd2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae
Co2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae
Mg2+ true substrate is the magnesium complexes of ATP Saccharomyces cerevisiae
Mn2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae
Ni2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae
Zn2+ ZnATP2- is the true substrate, free metal ions inhibit Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate rapid equilibrium random mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
850
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae ADP + 1,3-diphosphoglycerate
-
 r

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Saccharomyces cerevisiae
ATP true substrate is the magnesium complexes of ATP Saccharomyces cerevisiae
ATP required as phosphate donor Saccharomyces cerevisiae