Literature summary for 2.7.2.11 extracted from

Chen, M.; Cao, J.; Zheng, C.; Liu, Q.
Directed evolution of an artificial bifunctional enzyme, gamma-glutamyl kinase/gamma-glutamyl phosphate reductase, for improved osmotic tolerance of Escherichia coli transformants (2006), FEMS Microbiol. Lett., 263, 41-47.

Search for more literature for 2.7.2.11

Application

Application	Comment	Organism
additional information	proBA fusion gene and some of the mutated proBA fusion genes can be expressed and functionally complement the proline auxotrophy in Escherichia coli JM83, the fused gamma-glutamyl kinase/gamma-glutamyl phosphate reductase is more active than the separate gamma-glutamyl kinase and gamma-glutamyl phosphate reductase enzymes	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
proBA fusion gene and mutated proBA fusion gene expressed in Escherichia coli JM83	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
N177D	mutant of the proBA fusion gene, improves the osmotolerance of host cells of Escherichia coli JM83, leads to overproduction of proline by host cells, is about 100fold less sensitive to proline-mediated feedback inhibition than the control	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
L-proline	completely inhibits the activity of wild-type gamma-glutamyl kinase/gamma-glutamyl phosphate reductase at concentrations greater than 0.1mM, and inhibits it by 30% at 0.01mM	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	Q6E235	-	-
Bacillus subtilis 93151-14	Q6E235	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamate	-	Bacillus subtilis	ADP + L-glutamate 5-phosphate	-	?
ATP + L-glutamate	-	Bacillus subtilis 93151-14	ADP + L-glutamate 5-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
gamma-GK	-	Bacillus subtilis
gamma-glutamyl kinase	-	Bacillus subtilis

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Release 2023.1 (January 2023)