D156A |
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity |
Synechocystis sp. |
G123A |
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity |
Synechocystis sp. |
additional information |
construction of a fused sensor, Hik2n-Hik7c, which has the signal input domain of Hik2 and the kinase domain of the phosphate-deficiency sensor Hik7. The coding region of the hik7 gene is replaced with the fused sensor to evaluate the signalling activity in vivo as the activity of alkaline phosphatase (AP), which is regulated by Hik7. Cells expressing Hik2n-Hik7c have weak AP activities under standard growth conditions. Saline stress by NaCl induces AP activity in a dose-dependent manner. Hik2n-Hik7c responds to Cl- concentration. Amino acid substitutions in the signal input domain of Hik2 all result in loss of this responsiveness, i.e. mutants Q22A, P114A, G123A, R129K, W134F, Q141A and D156A completely lose AP activity |
Synechocystis sp. |
P114A |
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity |
Synechocystis sp. |
Q141A |
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity |
Synechocystis sp. |
Q22A |
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2nHik7c construct is inactive in alkaline phosphatase activity |
Synechocystis sp. |
R129K |
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity |
Synechocystis sp. |
W134F |
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity |
Synechocystis sp. |