Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H398L | the mutant retains itscapability to bind ATP and is competent to catalyze the transphosphorylation of an AtoS G-box (G565A) mutant protein which otherwise fails to autophosphorylate due to its inability to bind ATP | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HCl | cytoplasmic phosphorylated AtoS is sensitive to treatment with 1 N HCl but stable in the presence of 1 N NaOH | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation, histidine-398 in the conserved H-box is the phospho-accepting site | Escherichia coli |
Purification (Comment) | Organism |
---|---|
IMAC and Ni2+-NTA agarose column chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
protein refolding is achieved by dialysis in storage buffer (50 mM Tris-HCl pH 7.6, 0.5 mM dithiothreitol, 50% (v/v) glycerol) containing 0.01% (v/v) Triton X-100 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein L-histidine | - |
Escherichia coli | ADP + protein N-phospho-L-histidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AtoS | - |
Escherichia coli |
AtoS-AtoC two-component signal transduction system | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |