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Literature summary for 2.7.13.1 extracted from

  • Huang, J.; Wei, Y.K.; Osterberg, L.; Matthews, H.R.
    Purification of a protein histidine kinase from the yeast Saccharomyces cerevisiae. The first member of this class of protein kinases (1991), J. Biol. Chem., 266, 9023-9031.
    View publication on PubMed
Search for more literature for 2.7.13.1

Inhibitors

Inhibitors Comment Organism Structure
KCl
-
 Saccharomyces cerevisiae
NaCl
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 histone H4
-
 Saccharomyces cerevisiae
0.06
-
 ATP ATP in form of MgATP2- Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 2 mM, activation Saccharomyces cerevisiae
Mg2+ 15-20 mM, activation Saccharomyces cerevisiae
Mn2+ 1.5 mM, activation Saccharomyces cerevisiae
additional information not activated by Ca2+, Zn2+, Fe2+, Cu2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone H4
-
 Saccharomyces cerevisiae ADP + phosphohistone H4 phosphorylation of His 75 ?
ATP + histone H4 L-histidine
-
 Saccharomyces cerevisiae ADP + histone H4 Npi-phospho-L-histidine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Saccharomyces cerevisiae