Application | Comment | Organism |
---|---|---|
medicine | phosphorylation and regulation of the oncogene E7 of human papillomvirus HPV16 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
transfected into NIH-3T3 cells | Homo sapiens |
wild-type and mutant DNA fragments of DYRK1A, vectors pCMV-Tag2B, pRK5, pCITE4, pGEX-5X-1, pCDNA3-HA | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K188R | kinase-dead DYRK1 mutant | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13627 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CaSki cell | transfection analysis | Homo sapiens | - |
NCI-H1299 cell | transfection analysis | Homo sapiens | - |
U2-OS cell | transfection analysis, degradation of the tumor suppressor pRb in a dose-dependent manner based on the accumulation of HPV16E7 generated by DYRK1A transfection | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
stabilization of viral oncoproteins E6 and E7 of HPV16 by phosphorylation the threonine 5 and threonine 7 residues, substitution mutations, in vitro and in vivo interaction with HPV16E7, half-life of E7 protein extended by replacing threonine 5 and 7 residues with aspartic acid, DYRK1A-induced phosphorylation protects E7 from degradation, as a consequence inactivation of the tumor suppressor pRb is increased | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | phosphorylation of viral protein E7 of human papillomvirus HPV16, DYRK1A increases the transforming potential of HPV16-infected cells because of the greater stability of phosphorylated HPV16E7 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DYRK1A | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |