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Literature summary for 2.7.12.1 extracted from

  • Liang, Y.J.; Chang, H.S.; Wang, C.Y.; Yu, W.C.
    DYRK1A stabilizes HPV16E7 oncoprotein through phosphorylation of the threonine 5 and threonine 7 residues (2008), Int. J. Biochem. Cell Biol., 40, 2431-2441.
    View publication on PubMed

Application

Application Comment Organism
medicine phosphorylation and regulation of the oncogene E7 of human papillomvirus HPV16 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
transfected into NIH-3T3 cells Homo sapiens
wild-type and mutant DNA fragments of DYRK1A, vectors pCMV-Tag2B, pRK5, pCITE4, pGEX-5X-1, pCDNA3-HA Homo sapiens

Protein Variants

Protein Variants Comment Organism
K188R kinase-dead DYRK1 mutant Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13627
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS-PAGE Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
CaSki cell transfection analysis Homo sapiens
-
NCI-H1299 cell transfection analysis Homo sapiens
-
U2-OS cell transfection analysis, degradation of the tumor suppressor pRb in a dose-dependent manner based on the accumulation of HPV16E7 generated by DYRK1A transfection Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
stabilization of viral oncoproteins E6 and E7 of HPV16 by phosphorylation the threonine 5 and threonine 7 residues, substitution mutations, in vitro and in vivo interaction with HPV16E7, half-life of E7 protein extended by replacing threonine 5 and 7 residues with aspartic acid, DYRK1A-induced phosphorylation protects E7 from degradation, as a consequence inactivation of the tumor suppressor pRb is increased Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phosphorylation of viral protein E7 of human papillomvirus HPV16, DYRK1A increases the transforming potential of HPV16-infected cells because of the greater stability of phosphorylated HPV16E7 Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
DYRK1A
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens