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Literature summary for 2.7.12.1 extracted from
- Mutational analysis of stress-responsive peanut dual specificity protein kinase. Identification of tyrosine residues involved in regulation of protein kinase activity (2003), J. Biol. Chem., 278, 17328-17335.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is induced by abiotic stresses, the activation loop is located between subdomains VII and VIII, phosphorylation at Tyr317, Tyr297, and Tyr148 activates the enzyme | Arachis hypogaea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Arachis hypogaea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y148F | site-directed mutagenesis, ATP binding site residue, the mutant is unable to phosphorylate histone and is not phosphorylated itself | Arachis hypogaea |
| Y213F | site-directed mutagenesis, TEY motif residue, the mutant shows 4fold increased autophosphorylation and 2.8fold increased activity with ATP and histone compared to the wild-type enzyme | Arachis hypogaea |
| Y297F | site-directed mutagenesis, inactive mutant enzyme | Arachis hypogaea |
| Y317F | site-directed mutagenesis, ATP binding site residue, the mutant is unable to phosphorylate histone and is not phosphorylated itself | Arachis hypogaea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
histone H1 | recombinant mutant Y213F | Arachis hypogaea | |
| 0.007 | - |
histone H1 | recombinant wild-type enzyme | Arachis hypogaea | |
| 0.009 | - |
histone H1 | recombinant wild-type enzyme | Arachis hypogaea | |
| 0.011 | - |
histone H1 | recombinant mutants Y148F and Y297F | Arachis hypogaea | |
| 0.012 | - |
histone H1 | recombinant mutant Y317F | Arachis hypogaea | |
| 0.019 | - |
ATP | recombinant mutant Y213F | Arachis hypogaea |  |
| 0.034 | - |
ATP | recombinant wild-type enzyme | Arachis hypogaea | |
| 0.059 | - |
ATP | recombinant mutant Y317F | Arachis hypogaea | |
| 0.081 | - |
ATP | recombinant mutant Y297F | Arachis hypogaea | |
| 0.107 | - |
ATP | recombinant mutant Y148F | Arachis hypogaea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arachis hypogaea | the enzyme is developmentally regulated | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arachis hypogaea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | MALDI-MS phosphorylation site mapping, the enzyme performs autophosphorylation within the ATP binding site at Tyr148 and Tyr317, at Tyr213 of the activation loop, and at Tyr297 in the C-terminus | Arachis hypogaea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arachis hypogaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + histone H1 | - |
Arachis hypogaea | ADP + phosphorylated histone H1 | - |
? | |
| additional information | the enzyme is developmentally regulated | Arachis hypogaea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | analysis of sequence motifs | Arachis hypogaea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| serine/threonine/tyrosine protein kinase | - |
Arachis hypogaea |
| STY protein kinase | - |
Arachis hypogaea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
ATP | recombinant mutant Y317F | Arachis hypogaea | |
| 0.54 | - |
ATP | recombinant mutant Y297F | Arachis hypogaea | |
| 1.02 | - |
ATP | recombinant mutant Y148F | Arachis hypogaea | |
| 3.09 | - |
histone H1 | recombinant mutant Y148F | Arachis hypogaea | |
| 3.3 | - |
histone H1 | recombinant mutant Y317F | Arachis hypogaea | |
| 3.71 | - |
histone H1 | recombinant mutant Y297F | Arachis hypogaea | |
| 4.74 | - |
histone H1 | recombinant mutant Y317F | Arachis hypogaea | |
| 5.25 | - |
ATP | recombinant wild-type enzyme | Arachis hypogaea | |
| 14.5 | - |
ATP | recombinant mutant Y213F | Arachis hypogaea | |
| 18.4 | - |
histone H1 | recombinant mutant Y213F | Arachis hypogaea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | binding site residue Tyr317 is important | Arachis hypogaea | |
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