Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | - |
Dictyostelium discoideum |
Cloned (Comment) | Organism |
---|---|
expression of MHCK A, comprising residues 527-887, i.e. A-CAT, as a GST-fusion protein, phylogenetic analysis | Dictyostelium discoideum |
expression of MHCK B, comprising residues 13-459, i.e. B-CAT, as a GST-fusion protein, phylogenetic analysis | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Dictyostelium discoideum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin heavy-chain | Dictyostelium discoideum | - |
ADP + myosin heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | P42527 | MHCK A | - |
Dictyostelium discoideum | P90648 | MHCK B | - |
Purification (Comment) | Organism |
---|---|
recombinant MHCK A GST-tagged catalytic domain by glutathione affinity chromatography | Dictyostelium discoideum |
recombinant MHCK B GST-tagged catalytic domain by glutathione affinity chromatography | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin heavy-chain | - |
Dictyostelium discoideum | ADP + myosin heavy chain phosphate | - |
? | |
additional information | substrate specificity of the MHCK A catalytic domain: a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by MHCK A. Phosphorylation by MHCK A is enhanced 2 to 4fold by basic residues in the P+2, P+3 and P+4 positions, with a strong preference for threonine as the phosphoacceptor amino acid, overview | Dictyostelium discoideum | ? | - |
? | |
additional information | substrate specificity of the MHCK B catalytic domain: a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by MHCK B. Phosphorylation by MHCK B is further enhanced 8fold by a basic residue in the P+2 position, strong preference for threonine as the phosphoacceptor amino acid, overview | Dictyostelium discoideum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MHCK A | - |
Dictyostelium discoideum |
MHCK B | - |
Dictyostelium discoideum |
myosin II heavy chain kinases A | - |
Dictyostelium discoideum |
myosin II heavy chain kinases B | - |
Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Dictyostelium discoideum |