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Literature summary for 2.7.11.7 extracted from

  • Egelhoff, T.T.; Croft, D.; Steimle, P.A.
    Actin activation of myosin heavy chain kinase A in Dictyostelium: a biochemical mechanism for the spatial regulation of myosin II filament disassembly (2005), J. Biol. Chem., 280, 2879-2887.
    View publication on PubMed
Search for more literature for 2.7.11.7

Activating Compound

Activating Compound Comment Organism Structure
F-actin about 40fold activation of myosin heavy chain kinase A, structure and mechanism, stimulation via enzyme autophosphorylation Dictyostelium discoideum
G-actin slight activation of MHCK A, activation is abolished by latrunculin A, which depolymerizes the actin filament Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged full-length isozyme MHCK A and truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Dictyostelium discoideum, overexpression of isolated coiled-coil domain and of the catalytic domain in Escherichia coli as GST-fused proteins Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
additional information the truncation mutant DELTAcoil-MHCK A comprising residues 1-498 is not affected in its autophosphorylation rate by F-actin Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Dictyostelium discoideum
KCl activates Dictyostelium discoideum
Mg2+
-
 Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin-heavy-chain] Dictyostelium discoideum
-
 ADP + [myosin-heavy-chain]phosphate
-
 ?
additional information Dictyostelium discoideum biochemical mechanism for the spatial regulation of myosin II filament disassembly acts via MHCK A activation by actin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum P42527 isozyme MHCK A; isozyme MHCK A
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein MHCK A performs autophosphorylation, facilitated by F-actin requiring the coiled coil domain of the enzyme Dictyostelium discoideum

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged full-length isozyme MHCK A and truncation mutant DELTAcoil-MHCK A from Dictyostelium discoideum to homogeneity by affinity chromatography, recombinant GST-fusion coiled-coil domain and catalytic domain from Escherichia coli to near homogeneity by affinity chromatography Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin-heavy-chain]
-
 Dictyostelium discoideum ADP + [myosin-heavy-chain]phosphate
-
 ?
additional information biochemical mechanism for the spatial regulation of myosin II filament disassembly acts via MHCK A activation by actin Dictyostelium discoideum ?
-
 ?
additional information MHCK A performs autophosphorylation Dictyostelium discoideum ?
-
 ?

Synonyms

Synonyms Comment Organism
MHCK A
-
 Dictyostelium discoideum
myosin heavy chain kinase A
-
 Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Dictyostelium discoideum