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Literature summary for 2.7.11.7 extracted from
- Identification of three phosphorylation sites on each heavy chain of Acanthamoeba myosin II (1981), J. Biol. Chem., 256, 12811-12816.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+/calmodulin | weak | Acanthamoeba castellanii | |
| KCl | 60% inhibition at 0.1 M | Acanthamoeba castellanii |  |
| additional information | no inhibition by cAMP or cGMP | Acanthamoeba castellanii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Acanthamoeba castellanii | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | not required | Acanthamoeba castellanii | |
| Mg2+ | requirement | Acanthamoeba castellanii | |
| Mg2+ | cannot be substituted by Ca2+ or Mn2+ | Acanthamoeba castellanii | |
| additional information | no activation by Mn2+ | Acanthamoeba castellanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin II heavy chain | Acanthamoeba castellanii | - |
ADP + myosin II heavy chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba castellanii | - |
myosin II heavy chain kinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Acanthamoeba castellanii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Acanthamoeba castellanii | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, several months | Acanthamoeba castellanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin II heavy chain | - |
Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | myosin light chains are no substrates | Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | incorporation of 3 mol phosphate per mol of myosin II heavy chain, phosphorylation sites are mainly identical in vitro and in vivo | Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | phosphorylates serine residues | Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
| additional information | the Mg2+-ATPase activity of the substrate myosin II is inhibited in vitro by its phosphorylation | Acanthamoeba castellanii | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Acanthamoeba castellanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Acanthamoeba castellanii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Acanthamoeba castellanii | |
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Release 2023.1 (January 2023)
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