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Literature summary for 2.7.11.7 extracted from

  • Medley, Q.W.; Lee, S.F.; Cote, G.P.
    Purification and characterization of myosin II heavy chain kinase A from Dictyostelium (1991), Methods Enzymol., 196, 23-34.
    View publication on PubMed
Search for more literature for 2.7.11.7

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is stimulated by its autophosphorylation Dictyostelium discoideum

General Stability

General Stability Organism
loses about 25% of activity for every freeze-thaw cycle Dictyostelium discoideum
phosphorylated enzyme is somewhat less stable than unphosphorylated enzyme Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
additional information ionic strength has no effect on autophosphorylation activity; strong inhibition of phosphorylation activity by increasing ionic strength Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 RKKFGESEKTKTKEFL-amide pH 7.5, 25°C Dictyostelium discoideum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Dictyostelium discoideum
Mg2+ absolutely requires 1-2 mM Mg2+ Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 x * 130000, SDS-PAGE Dictyostelium discoideum
700000
-
 above, gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin I heavy chain Dictyostelium discoideum
-
 ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain Dictyostelium discoideum Ax-3
-
 ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin II heavy chain Dictyostelium discoideum
-
 ADP + myosin II heavy chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Dictyostelium discoideum
-
 isozyme myosin II heavy chain kinase A
-
Dictyostelium discoideum Ax-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isolation of highly phosphorylated and unphosphorylated myosin II heavy chain kinase A Dictyostelium discoideum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.98
-
 purified enzyme Dictyostelium discoideum
0.98
-
 substrate myosin II heavy chain Dictyostelium discoideum

Storage Stability

Storage Stability Organism
0°C, highly purified preparation of unphosphorylated enzyme, 25% loss of activity per day Dictyostelium discoideum
0°C, partially purified preparation of unphosphorylated enzyme, several days Dictyostelium discoideum
indefinitely stable upon storage in liquid nitrogen, recovery of 60-70% activity after thawing Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + myosin heavy chain kinase intramolecular autophosphorylation Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin heavy chain kinase enzyme incorporates 2-3 mol phosphate per mol of 130 kDa subunit Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin I heavy chain
-
 Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain phosphorylation of residues Thr1833 and Thr2029 Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain 10 mol phosphate per mol of kinase subunit Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain 6-8 mol phosphate per mol of enzyme Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain
-
 Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain phosphorylation of residues Thr1833 and Thr2029 Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain 10 mol phosphate per mol of kinase subunit Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain 6-8 mol phosphate per mol of enzyme Dictyostelium discoideum Ax-3 ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin II heavy chain
-
 Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
 ?
ATP + myosin II heavy chain specific isozyme A for the heavy chain of myosin II Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
 ?
ATP + RKKFGESEKTKTKEFL-amide isozyme myosin II heavy chain kinase A Dictyostelium discoideum ?
-
 ?
ATP + RKKFGESEKTKTKEFL-amide synthetic peptide MH-3 Dictyostelium discoideum ?
-
 ?
ATP + synthetic peptides
-
 Dictyostelium discoideum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 130000, SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Dictyostelium discoideum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 1-2 h, stable Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Dictyostelium discoideum