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Literature summary for 2.7.11.4 extracted from
- Regulation of the branched chain 2-oxoacid dehydrogenase kinase reaction (1982), FEBS Lett., 144, 57-62.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoisocaproate | - |
Bos taurus |  |
| 3-methyl-2-oxobutanoate | - |
Bos taurus | |
| 4-methyl-2-oxopentanoate | - |
Bos taurus | |
| ADP | - |
Bos taurus | |
| branched-chain 2-oxo acids | - |
Bos taurus | |
| diphosphate | - |
Bos taurus | |
| MgATP2- | - |
Bos taurus | |
| additional information | no inhibition by acetyl-CoA; no inhibition by coenzyme A | Bos taurus | |
| thiamine | - |
Bos taurus | |
| thiamine diphosphate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0126 | - |
ATP | pH 7.5, 30°C | Bos taurus | |
| 0.0126 | - |
ATP | ATP in form of MgATP2- | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement, actual substrate: MgATP2- | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | Bos taurus | phosphorylation inactivates EC 1.2.4.4 | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] | - |
Bos taurus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | phosphorylates alpha-subunit of multienzyme complex component E1 | Bos taurus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | Ser-residues of MW 46000-subunit | Bos taurus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | phosphorylation inactivates EC 1.2.4.4 | Bos taurus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
loss of activity during purification at pH-values below 7 | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00027 | - |
ADP | pH 7.5, 30°C | Bos taurus | |
| 0.00048 | - |
4-methyl-2-oxopentanoate | pH 7.5, 30°C | Bos taurus | |
| 0.00092 | - |
2-oxoisocaproate | pH 7.5, 30°C | Bos taurus | |
| 0.004 | - |
diphosphate | pH 7.5, 30°C | Bos taurus | |
| 0.0059 | - |
thiamine | pH 7.5, 30°C | Bos taurus | |
| 0.0089 | - |
4-methyl-2-oxopentanoate | pH 7.5, 30°C | Bos taurus | |
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Release 2023.1 (January 2023)
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