Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Rattus norvegicus | |
AMP | 4.4fold activation at up to 0.08 mM | Homo sapiens | |
additional information | phosphorylation of AMPK activates the enzyme | Homo sapiens | |
additional information | phosphorylation of AMPK activates the enzyme | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of GST-tagged wild-type AMPK beta1-alpha1 subunits fusion protein in COS-7 cells, and expression of beta1 residue 367 mutants in fusion to gamma1 subunit in COS-7 cells | Rattus norvegicus |
expression of His6-tagged beta1(186-270)gamma1 complex and of GST-tagged isozyme AMPK alpha1beta1gamma1 in Spodoptera frugiperda Sf21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y267A | the mutation almost completely abrogates the beta1gamma1 interaction in comparison to wild type enzyme | Rattus norvegicus |
Y267F | the mutation affects the beta1gamma1 interaction in comparison to wild type enzyme | Rattus norvegicus |
Y267H | the mutation affects the beta1gamma1 interaction in comparison to wild type enzyme | Rattus norvegicus |
Y267S | the mutation affects the beta1gamma1 interaction in comparison to wild type enzyme | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation at alpha1-Thr172 by CaMKKbeta, and at alpha1-Ser485, beta1-Ser108, and beta1-Ser182 in Sf21 cells, determination of phosphorylation sites by mass spectrometry, phosphorylation also by LKB1, overview | Homo sapiens |
phosphoprotein | phosphorylation by CaMKKbeta and LKB1, determination of phosphorylation sites by mass spectrometry, overview | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged isozyme AMPK alpha1beta1gamma1 from Spodoptera frugiperda Sf21 cells by glutathione affinity and anion exchange chromatography, and gel filtration | Homo sapiens |
recombinant GST-tagged isozyme AMPK beta1-alpha1 subunits fusion protein from COS-7 cells by glutathione affinity exchange chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
purified recombinant enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [peptide HMRSAMSGLHLVKRR] | i.e. SAMS peptide | Homo sapiens | ADP + [peptide HMRSAMSGLHLVKRR] phosphate | - |
? | |
ATP + [peptide HMRSAMSGLHLVKRR] | i.e. SAMS peptide | Rattus norvegicus | ADP + [peptide HMRSAMSGLHLVKRR] phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | AMPK consists of a catalytic alpha subunit and two non-catalytic subunits,beta and gamma, each with multiple isoforms that form active 1:1:1 heterotrimers | Homo sapiens |
More | AMPK consists of a catalytic alpha subunit and two non-catalytic subunits,beta and gamma, each with multiple isoforms that form active 1:1:1 heterotrimers, beta1-Thr263 and beta1-Tyr267 are required for beta1gamma1 subunit association | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
AMP-activated protein kinase | - |
Homo sapiens |
AMP-activated protein kinase | - |
Rattus norvegicus |
AMPK | - |
Homo sapiens |
AMPK | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus |