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Literature summary for 2.7.11.30 extracted from
- Semisynthesis of phosphovariants of Smad2 reveals a substrate preference of the activated TbetaRI kinase (2004), Biochemistry, 43, 5698-5706.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SARA protein | i.e. Smad anchor for receptor activation protein, binding site for co-modulator Smad4, modulates self-association of partially phosphorylated Smad2 preventing premature release of monophosphorylated substrate, interaction with Smad2 via the Smad2 MH2 domain | Homo sapiens | |
| TGF-beta | binds to and activates TbetaRI receptor kinase that phosphorylates Smad2 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
| Mn2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Smad2 | Homo sapiens | TGF-beta-induced phosphorylation by TbetaRI receptor kinase at both phosphorylation sites Ser465 and Ser467 leads to release of Smad2 from membrane-anchored protein SARA and signaling co-mediator Smad4, translocation into the nucleus, and regulation of target gene expression | ADP + phosphorylated Smad2 | - |
? | |
| additional information | Homo sapiens | TGF-beta signaling is involved in a wide range of cellular processes and various disease states in humans, R-Smad phosphorylation plays a key role | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P36897 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + KMGSPSVRCS(P)SMS | TGF-beta-induced phosphorylation by TbetaRI receptor kinase of the Smad2-derived, phosphorylated peptide substrate containing Ser465 phosphorylation site, poor activity with nonphosphorylated peptide substrate | Homo sapiens | ADP + ? | - |
? | |
| ATP + Smad2 | TGF-beta-induced phosphorylation by TbetaRI receptor kinase at both phosphorylation sites Ser465 and Ser467 leads to release of Smad2 from membrane-anchored protein SARA and signaling co-mediator Smad4, translocation into the nucleus, and regulation of target gene expression | Homo sapiens | ADP + phosphorylated Smad2 | - |
? | |
| ATP + Smad2 | TGF-beta-induced phosphorylation by TbetaRI receptor kinase at two phosphorylation sites Ser465 and Ser467 within the MH2 domain, activity with Smad2 analogues, overview | Homo sapiens | ADP + phosphorylated Smad2 | - |
? | |
| additional information | TGF-beta signaling is involved in a wide range of cellular processes and various disease states in humans, R-Smad phosphorylation plays a key role | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TbetaRI kinase | - |
Homo sapiens |
| TbetaRI receptor kinase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 30 | assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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