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Literature summary for 2.7.11.27 extracted from

  • Shiao, M.S.; Drong, R.F.; Porter, J.W.
    The purification and properties of a protein kinase and the partial purification of a phosphoprotein phosphatase that inactivate and activate acetyl-CoA carboxylase (1981), Biochem. Biophys. Res. Commun., 98, 80-87.
    View publication on PubMed
Search for more literature for 2.7.11.27

Activating Compound

Activating Compound Comment Organism Structure
ATP required Rattus norvegicus
additional information cAMP independent Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 SDS-PAGE, two lighter bands at 40000 and 60000 Da detected Rattus norvegicus
160000 200000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [acetyl-CoA carboxylase] Rattus norvegicus phosphorylates and inactivates acetyl-CoA carboxylase, (EC 6.4.1.2), and is therefore involved in regulation of long chain fatty acid synthesis ADP + [acetyl-CoA carboxylase] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Holtzman rats
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, partially purified kinase preparation, stable for several months, highly purified kinase is very labile and loses activity within 5 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [acetyl-CoA carboxylase] inactivation of carboxylase Rattus norvegicus ADP + [acetyl-CoA carboxylase] phosphate
-
 ?
ATP + [acetyl-CoA carboxylase] phosphorylates and inactivates acetyl-CoA carboxylase, (EC 6.4.1.2), and is therefore involved in regulation of long chain fatty acid synthesis Rattus norvegicus ADP + [acetyl-CoA carboxylase] phosphate
-
 ?