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Literature summary for 2.7.11.25 extracted from

  • Witowsky, J.A.; Johnson, G.L.
    Ubiquitylation of MEKK1 inhibits its phosphorylation of MKK1 and MKK4 and activation of the ERK1/2 and JNK pathways (2003), J. Biol. Chem., 278, 1403-1406.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzyme Mus musculus

Protein Variants

Protein Variants Comment Organism
C441A site-directed mutagenesis, the mutant enzyme is not ubiquitinylated and thus shows a higher ERK activating activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information ubiquitinylation of MEKK1 inhibits the enzyme in vitro and in vivo Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + MKK1 Mus musculus MKK1 activates the ERK2 signaling pathway ADP + phosphorylated MKK1
-
?
ATP + MKK4 Mus musculus MKK4 activates the JNK signaling pathway ADP + phosphorylated MKK4
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the active MEKK1 stimulates its own ubiquitinylation in vivo which has a negatively regulating function Mus musculus
proteolytic modification cleavage by caspase-3 at a specific C-terminal cleavage site generates a 91 kDa, catalytically active enzyme fragment Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + MKK1 MKK1 activates the ERK2 signaling pathway Mus musculus ADP + phosphorylated MKK1
-
?
ATP + MKK1 a MAPKK, recombinant GST-tagged MKK1 Mus musculus ADP + phosphorylated MKK1
-
?
ATP + MKK4 MKK4 activates the JNK signaling pathway Mus musculus ADP + phosphorylated MKK4
-
?
ATP + MKK4 a MAPKK, recombinant GST-tagged MKK4 Mus musculus ADP + phosphorylated MKK4
-
?

Subunits

Subunits Comment Organism
More the C-terminus contains the kinase domain, two ubiquitin interaction motifs, a region of interaction with small GTPases, and a caspase 3-like cleavage site Mus musculus

Synonyms

Synonyms Comment Organism
MEKK1
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus