Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzyme | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C441A | site-directed mutagenesis, the mutant enzyme is not ubiquitinylated and thus shows a higher ERK activating activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | ubiquitinylation of MEKK1 inhibits the enzyme in vitro and in vivo | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK1 | Mus musculus | MKK1 activates the ERK2 signaling pathway | ADP + phosphorylated MKK1 | - |
? | |
ATP + MKK4 | Mus musculus | MKK4 activates the JNK signaling pathway | ADP + phosphorylated MKK4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the active MEKK1 stimulates its own ubiquitinylation in vivo which has a negatively regulating function | Mus musculus |
proteolytic modification | cleavage by caspase-3 at a specific C-terminal cleavage site generates a 91 kDa, catalytically active enzyme fragment | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK1 | MKK1 activates the ERK2 signaling pathway | Mus musculus | ADP + phosphorylated MKK1 | - |
? | |
ATP + MKK1 | a MAPKK, recombinant GST-tagged MKK1 | Mus musculus | ADP + phosphorylated MKK1 | - |
? | |
ATP + MKK4 | MKK4 activates the JNK signaling pathway | Mus musculus | ADP + phosphorylated MKK4 | - |
? | |
ATP + MKK4 | a MAPKK, recombinant GST-tagged MKK4 | Mus musculus | ADP + phosphorylated MKK4 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminus contains the kinase domain, two ubiquitin interaction motifs, a region of interaction with small GTPases, and a caspase 3-like cleavage site | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
MEKK1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |