Cloned (Comment) | Organism |
---|---|
expression of HA-tagged active MEKK2 and inactive S519A MEKK2 mutant catalytic sites, expression of wild-type and mutant full length MEKK2s and of wild-type and mutant MEKK3s | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
S519A | site-directed mutagenesis, MEKK2 phosphorylation site mutant, inactive mutant | Mus musculus |
S526A | site-directed mutagenesis, MEKK3 phosphorylation site mutant, inactive mutant | Mus musculus |
T521A | site-directed mutagenesis, MEKK2 phosphorylation site mutant, the mutant shows slightly reduced activity compared to the wild-type enzyme | Mus musculus |
T523A | site-directed mutagenesis, MEKK2 phosphorylation site mutant, the mutant shows slightly reduced activity compared to the wild-type enzyme | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | MEKK2 and MEKK3 are involved in activation of signal transduction pathways via toll-like receptor TLR, mitogen-activated protein kinases MAPK, and NF-kappaB, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | MEKK2 and MEKK3 are activated by phosphorylation, determination of phosphorylation sites, S519 is a key regulator phosphorylation site in MEKK2, other sites are Thr521 and Thr523 | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | molecular mechanism of MEKK2 and MEKK3 | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MEKK2 and MEKK3 are involved in activation of signal transduction pathways via toll-like receptor TLR, mitogen-activated protein kinases MAPK, and NF-kappaB, overview | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAP3K | - |
Mus musculus |
MEKK2 | - |
Mus musculus |
MEKK3 | - |
Mus musculus |
More | MEKK2 and MEKK3 belong to the MEKK/Ste11 subfamily of the MAP3K family of enzymes | Mus musculus |