Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.25 extracted from

  • Huang, J.; Tu, Z.; Lee, F.S.
    Mutations in protein kinase subdomain X differentially affect MEKK2 and MEKK1 activity (2003), Biochem. Biophys. Res. Commun., 303, 532-540.
    View publication on PubMed
Search for more literature for 2.7.11.25

Cloned(Commentary)

Cloned (Comment) Organism
MEKK2, DNA and amino acid sequence determination, expression of wild-type and mutant MEKK2 by reticulocyte lysate TnT T7 mixture, expression of wild-type and mutant MEKK2 and MEKK1 in COS-1 cells and in HeLa cells, coexpression with ERK5 in COS-1 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
F1443A site-directed mutagenesis of MEKK1, the mutation abolishes the in vitro interaction with MKK4, but retains the in vivo activity Mus musculus
F571A site-directed mutagenesis of MEKK2, inactive mutant Mus musculus
I1445A site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function Mus musculus
I1454A site-directed mutagenesis of MEKK1, the mutation abolishes the in vitro interaction with MKK4, but retains the in vivo activity Mus musculus
I573A site-directed mutagenesis of MEKK2, inactive mutant Mus musculus
L1458A site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function Mus musculus
L582A/P583A site-directed mutagenesis of MEKK2, inactive mutant Mus musculus
additional information mutations in the MEKK subdomain X differentially affect MEKK2 and MEKK1 activity, overview Mus musculus
P1452A site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function Mus musculus
P1455A site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function Mus musculus
P580A site-directed mutagenesis of MEKK2, the mutant shows 82% of wild-type activity Mus musculus
P584A site-directed mutagenesis of MEKK2, the mutant shows activity similar to the wild-type enzyme Mus musculus
S1459A site-directed mutagenesis of MEKK1, the mutation abolishes the in vitro interaction with MKK4, but retains the in vivo activity Mus musculus
T575A/Q576A/P577A site-directed mutagenesis of MEKK2, inactive mutant Mus musculus
V586A site-directed mutagenesis of MEKK2, the mutant shows activity similar to the wild-type enzyme Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + MEK5 Mus musculus activated MEK5 activates ERK5 ADP + phosphorylated MEK5
-
 ?
ATP + MKK4 Mus musculus a MAPKK ADP + phosphorylated MKK4
-
 ?
additional information Mus musculus MEKK2 induces the JNK signaling pathway, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + MEK5 a MAPKK Mus musculus ADP + phosphorylated MEK5
-
 ?
ATP + MEK5 activated MEK5 activates ERK5 Mus musculus ADP + phosphorylated MEK5
-
 ?
ATP + MKK4 a MAPKK Mus musculus ADP + phosphorylated MKK4
-
 ?
additional information MEKK2 induces the JNK signaling pathway, overview Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
More the subdomain X is required for phosphorylation and activation of MAPKKs in vivo Mus musculus

Synonyms

Synonyms Comment Organism
MEKK1
-
 Mus musculus
MEKK2
-
 Mus musculus
MEKK3
-
 Mus musculus
MEKK4
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus