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Literature summary for 2.7.11.24 extracted from

  • Zhang, Y.Y.; Mei, Z.Q.; Wu, J.W.; Wang, Z.X.
    Enzymatic activity and substrate specificity of mitogen-activated protein kinase p38alpha in different phosphorylation states (2008), J. Biol. Chem., 283, 26591-26601.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.24

Activating Compound

Activating Compound Comment Organism Structure
additional information full activation of the MAP kinases requires dual phosphorylation of the Thr and Tyr residues in the TXY motif of the activation loop by MAP kinase kinases, p38alpha phosphorylated at both Thr180 and Tyr182 is 10-20fold more active than p38alpha phosphorylated at Thr180 only, p38alpha phosphorylation by MKK6 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged p38alpha in Escherichia coli strain BL21(DE3) Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information downregulation of MAP kinase activity can be initiated by dephosphorylation through multiple serine/threonine phosphatases, tyrosine-specific phosphatases, and dual specificity phosphatases, i.e. MAP kinase phosphatases, leading to the formation of monophosphorylated MAP kinases Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 ATF2DELTA109 pH 7.0, 25°C, biphosphorylated p38alpha Mus musculus
0.02
-
 ATF2DELTA109 pH 7.0, 25°C, monophosphorylated p38alpha Mus musculus
0.656
-
 EGF receptor peptide pH 7.0, 25°C, biphosphorylated p38alpha Mus musculus
2.8
-
 EGF receptor peptide pH 7.0, 25°C, monophosphorylated p38alpha Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus MAP kinases are essential signaling molecules that mediate many cellular effects of growth factors, cytokines, and stress stimuli ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein full activation of the MAP kinases requires dual phosphorylation of the Thr and Tyr residues in the TXY motif of the activation loop by MAP kinase kinases. Dephosphorylation of MAP kinases by phosphatases, e.g. multiple serine/threonine phosphatases, tyrosine-specific phosphatases, and dual specificity phosphatases, leads to formation of monophosphorylated enzymes and inhibition of the MAP kinases. p38alpha phosphorylated at both Thr180 and Tyr182 is 10-20fold more active than p38alpha phosphorylated at Thr180 only, whereas p38alpha phosphorylated at Tyr182 alone is inactive, the dual-specific MKP5, the tyrosine-specific hematopoietic protein-tyrosine phosphatase, and the serine/threonine-specific PP2Calpha are all highly specific for the dephosphorylation of p38alpha, p38alpha phosphorylation by MKK6, kinetics, overview Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged p38alpha from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography to homogeneity Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ATF2DELTA109
-
 Mus musculus ADP + phosphorylated ATF2DELTA109
-
 ?
ATP + EGF receptor peptide
-
 Mus musculus ADP + phosphorylated EGF receptor peptide
-
 ?
additional information MAP kinases are essential signaling molecules that mediate many cellular effects of growth factors, cytokines, and stress stimuli Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
MAP kinase
-
 Mus musculus
More MAP kinase p38alpha is a member of the MAP kinase family Mus musculus
p38alpha
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at, p38alpha Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 ATF2DELTA109 pH 7.0, 25°C, monophosphorylated p38alpha Mus musculus
4.7
-
 ATF2DELTA109 pH 7.0, 25°C, biphosphorylated p38alpha Mus musculus
6.99
-
 EGF receptor peptide pH 7.0, 25°C, monophosphorylated p38alpha Mus musculus
31.6
-
 EGF receptor peptide pH 7.0, 25°C, biphosphorylated p38alpha Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, p38alpha Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus