Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | upon hyperosmotic stress, Hog1 is activated by phosphorylation | Saccharomyces cerevisiae | |
sorbitol | upon exposure to 1000 mM sorbitol, Hog1 is phosphorylated rapidly, with modification peaking at 10 to 20 min and then declining as cells adapt | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
additional information | a role for active Hog1 in maintaining signaling specificity under conditions of persistently high external osmolarity | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
into vector pCR2.1, expressed in Escherichia coli DH5alpha | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D144A | kinase-dead mutant, is unable to grow on 1000 mM sorbitol | Saccharomyces cerevisiae |
K52R | retains residual activity that permitts slow but readily detectable growth on 1000 mM sorbitol, residual growth is eliminated in the presence of 1-NM-PP1 | Saccharomyces cerevisiae |
additional information | hog1delta mutant, is unable to grow in the presence of 1-NM-PP1 | Saccharomyces cerevisiae |
T100A | Hog1-as-mutant, fully functional analog-sensitive allele of HOG1, is unable to grow in the presence of 1-NM-PP1, permits acute inhibition of the enzyme without other detectable perturbations of the cell, in the presence of inhibitor Rck2 modification is completely abrogated | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | wild-type Hog1 phosphorylation is unaffected by 1-NM-PP1 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | during growth under isosmotic conditions, presence of Hog1, the Hog1-asmutant, and mutants K52R and D144A | Saccharomyces cerevisiae | 5829 | - |
nucleus | within 10 min after exposure to 1000 mM sorbitol, Hog1 and Hog1-asmutant almost exclusively present in the nucleus, nuclear import of activated Hog1 requires its catalytic function | Saccharomyces cerevisiae | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Rck2 | - |
Saccharomyces cerevisiae | ADP + phosphorylated Rck2 | - |
? | |
additional information | kinase activity of Hog1 is required to promote its own dephosphorylation after hyperosmotic-stress-induced activation, moreover, catalytic activity of Hog1 is required continuously to prevent cross talk between the the high-osmolarity glycerol pathway and both the pheromone response and invasive growth pathways | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hog1 | - |
Saccharomyces cerevisiae |
MAPK | - |
Saccharomyces cerevisiae |
mitogen-activated protein kinase | - |
Saccharomyces cerevisiae |