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Literature summary for 2.7.11.24 extracted from

  • Bukhtiyarova, M.; Northrop, K.; Chai, X.; Casper, D.; Karpusas, M.; Springman, E.
    Improved expression, purification, and crystallization of p38alpha MAP kinase (2004), Protein Expr. Purif., 37, 154-161.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
p38alpha MAP kinase expression in Escherichia coli strain BL21(DE3) as His-tagged protein with a thrombin cleavage site Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant p38alpha MAP kinase free or in complex with inhibitor SB203580, sitting or hanging drop vapour diffusion method at 16-20°C, 16 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 10 mM MgCl2, 10 mM DTT, and 5% glycerol is mixed with reservoir solution containing 10-20% PEG 4000, 18% ethylene glycol, 0.1 M cacodylic acid, pH 6.0, at a volume ratio of 3:2, X-ray diffraction structure determination and analysis at 1.9-2.7 A resolution Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
SB203580 specific inhibitor of p38alpha MAP kinase, interaction analysis with immobilized enzyme in a surface plasmon resonance study, binding structure from the cyrstal structure of the enzyme-inhibitor complex Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
x * 64000, recombinant p38alpha MAP kinase, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
isozyme alpha of p38 MAP kinase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein p38alpha MAP kinase is phosphorylated by the MAPKK MKK6, EC 2.7.11.25, at Thr180 and Tyr182, phosphorylation activates p38alpha MAPK Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged p38alpha MAP kinase from Escherichia coli strain BL21(DE3) by nickel chelate affinity chromatohgraphy, dialysis, and ion exchange chromatography to homogeneity, the His-tag is cleaved off by thrombin Mus musculus

Subunits

Subunits Comment Organism
? x * 64000, recombinant p38alpha MAP kinase, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
p38alpha MAP kinase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Mus musculus