Cloned (Comment) | Organism |
---|---|
p38alpha MAP kinase expression in Escherichia coli strain BL21(DE3) as His-tagged protein with a thrombin cleavage site | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant p38alpha MAP kinase free or in complex with inhibitor SB203580, sitting or hanging drop vapour diffusion method at 16-20°C, 16 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 10 mM MgCl2, 10 mM DTT, and 5% glycerol is mixed with reservoir solution containing 10-20% PEG 4000, 18% ethylene glycol, 0.1 M cacodylic acid, pH 6.0, at a volume ratio of 3:2, X-ray diffraction structure determination and analysis at 1.9-2.7 A resolution | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SB203580 | specific inhibitor of p38alpha MAP kinase, interaction analysis with immobilized enzyme in a surface plasmon resonance study, binding structure from the cyrstal structure of the enzyme-inhibitor complex | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
x * 64000, recombinant p38alpha MAP kinase, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isozyme alpha of p38 MAP kinase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | p38alpha MAP kinase is phosphorylated by the MAPKK MKK6, EC 2.7.11.25, at Thr180 and Tyr182, phosphorylation activates p38alpha MAPK | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged p38alpha MAP kinase from Escherichia coli strain BL21(DE3) by nickel chelate affinity chromatohgraphy, dialysis, and ion exchange chromatography to homogeneity, the His-tag is cleaved off by thrombin | Mus musculus |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, recombinant p38alpha MAP kinase, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
p38alpha MAP kinase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Mus musculus |