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Literature summary for 2.7.11.22 extracted from

  • Menoyo, S.; Ricco, N.; Bru, S.; Hernandez-Ortega, S.; Escote, X.; Aldea, M.; Clotet, J.
    Phosphate-activated cyclin-dependent kinase stabilizes G1 cyclin to trigger cell cycle entry (2013), Mol. Cell. Biol., 33, 1273-1284.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.22

Activating Compound

Activating Compound Comment Organism Structure
cyclin Cln3 stability of Cln3 protein is diminished in strains with low activity of Pho85, a phosphate-sensing CDK. Cln3 is an in vitro substrate of Pho85, and both proteins interact in vivo. In vivo phosphorylation of S449 and T520 is essential to maintain Cln3 levels, Pho85 activity increases the half-life of Cln3 protein Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged Pho85 and Pho80 in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae Pho85 is a proline-directed kinase that preferentially phosphorylates the consensus sequence S/T-P-X-I/L. Cln3 contains two of these sites (Ser449 and Thr520) located precisely at the ends of the PEST region. Cln3 is an in vitro substrate of Pho85, and both proteins interact in vivo, Cln3 is a molecular target of the Pho85 kinase ?
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 ?
additional information Saccharomyces cerevisiae BY4741 Pho85 is a proline-directed kinase that preferentially phosphorylates the consensus sequence S/T-P-X-I/L. Cln3 contains two of these sites (Ser449 and Thr520) located precisely at the ends of the PEST region. Cln3 is an in vitro substrate of Pho85, and both proteins interact in vivo, Cln3 is a molecular target of the Pho85 kinase ?
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
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Saccharomyces cerevisiae BY4741
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged Pho85 and Pho80 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Cln3 protein recombinant His-tagged Cln3 protein is phosphorylated by Pho85/Pho80 complexes at S449 and T520. Cdc28 phosphorylates Cln3 to a greater extent than Pho85, probably because it phosphorylates Cln3 at more sites in the PEST region than Pho85 Saccharomyces cerevisiae ADP + Cln3 phosphoprotein
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 ?
ATP + Cln3 protein recombinant His-tagged Cln3 protein is phosphorylated by Pho85/Pho80 complexes at S449 and T520. Cdc28 phosphorylates Cln3 to a greater extent than Pho85, probably because it phosphorylates Cln3 at more sites in the PEST region than Pho85 Saccharomyces cerevisiae BY4741 ADP + Cln3 phosphoprotein
-
 ?
additional information Pho85 is a proline-directed kinase that preferentially phosphorylates the consensus sequence S/T-P-X-I/L. Cln3 contains two of these sites (Ser449 and Thr520) located precisely at the ends of the PEST region. Cln3 is an in vitro substrate of Pho85, and both proteins interact in vivo, Cln3 is a molecular target of the Pho85 kinase Saccharomyces cerevisiae ?
-
 ?
additional information Pho85 is a proline-directed kinase that preferentially phosphorylates the consensus sequence S/T-P-X-I/L. Cln3 contains two of these sites (Ser449 and Thr520) located precisely at the ends of the PEST region. Cln3 is an in vitro substrate of Pho85, and both proteins interact in vivo, Cln3 is a molecular target of the Pho85 kinase Saccharomyces cerevisiae BY4741 ?
-
 ?

Synonyms

Synonyms Comment Organism
Cdc28
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 Saccharomyces cerevisiae
PHO85
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 Saccharomyces cerevisiae
phosphate-activated cyclin-dependent kinase
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 Saccharomyces cerevisiae
phosphate-sensing CDK
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 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function the phosphate-activated cyclin-dependent kinase Pho85 stabilizes G1 cyclin to trigger cell cycle entry, Pho85 does not control Cln3 stability through regulation of autophagy. The activity of Pho85 is essential for accumulating Cln3 and for reentering the cell cycle after phosphate refeeding. Cln3 is a molecular target of the Pho85 kinase that is required to modulate cell cycle entry in response to environmental changes in nutrient availability Saccharomyces cerevisiae