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Literature summary for 2.7.11.22 extracted from

  • Cuomo, M.E.; Platt, G.M.; Pearl, L.H.; Mittnacht, S.
    Cyclin-cyclin-dependent kinase regulatory response is linked to substrate recognition (2011), J. Biol. Chem., 286, 9713-9725.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.22

Activating Compound

Activating Compound Comment Organism Structure
cyclin A required for CDK2 activity Homo sapiens
cyclin B
-
 Homo sapiens
cyclin D1
-
 Homo sapiens
cyclin D2
-
 Homo sapiens
cyclin D3
-
 Homo sapiens
additional information substrate phosphorylation by CDK6 with K-cyclin from Kaposi sarcoma herpesvirus, K-cyclin expression in U2OS cells leads to fragmentation of actin stress fibers. CDK-cyclin and CDK-inhibitor-cyclin interaction mutational analysis, overview. Analysis of the inhibitor p27KIP1 docking site on cyclins, overview Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
p27KIP1-cyclin A-CDK2 complex, X-ray diffraction structure determination and analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of single mutations in K-cyclin cluster A and of several truncation mutants of the K-cyclin, overview. Inability to overcome a p16INK4a-dependent cell cycle inhibition in cells expressing each of the K-cyclin mutants resembling cyclin A Human gammaherpesvirus 8
additional information loss of p27KIP1 (or p21CIP) binding to the mutant cyclin D2-CDK complex. Mutagenesis of the isostructural residues in clusters A and B of the cellular cyclinD2 to resemble those of wild-type Kaposi sarcoma herpesvirus K-cyclin and investigated the impact of these alterations on the activity of the cellular cyclin Homo sapiens
T177A site-directed mutagenesis of CDK6 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
E2F1 peptide 81PALGRPPVKRRLDLE95 Homo sapiens
KSLNRPFPDKIPELK
-
 Homo sapiens
additional information CDK-inhibitor-cyclin interaction mutational analysis, overview. Analysis of the inhibitor p27KIP1 docking site on cyclins, overview. Loss of p27KIP1 (or p21CIP) binding to the mutant cyclin D2-CDK complex, but not of p16INK4a Homo sapiens
additional information docking of CIP/KIP inhibitors and surface region involved, overview Human gammaherpesvirus 8
p16Ink4a as recombinant His-tagged protein Homo sapiens
p21CIP as recombinant GST-tagged protein Homo sapiens
p27Kip1 as recombinant GST-tagged protein, formation of p27KIP1-cyclin A-CDK2 complex, the inhibitor interacts with both the cyclin and the CDK, analysis of the p27KIP1 docking site on cyclins, overview. p27KIP1 binds to the cyclin in a shallow groove where the hydrophobic amino acids of the MRAIL helix make multiple van der Waals contacts with p27KIP1 Homo sapiens
pRb peptide 866SNPPKPLKKLRFDIE880 Homo sapiens
RXL motif-containing peptide addition of this peptide significantly reduces substrate phosphorylation by cyclin E-CDK2 and cyclin D2-CDK6 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Human gammaherpesvirus 8
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CALD1 protein reccombinant human GST-tagged substrate, with CDK6 Homo sapiens ADP + CALD1 phosphoprotein
-
 ?
ATP + RNA polymerase II C-terminal domain reccombinant GST-tagged substrate Homo sapiens ADP + phosphorylated RNA polymerase II C-terminal domain
-
 ?
ATP + RXL motif-containing peptide peptide sequence corresponding to amino acids 866-880 of RNA polymerase II C-terminal domain. Substrate phosphorylation by CDK6 with K-cyclin from Kaposi sarcoma herpesvirus, but addition of this peptide significantly reduces substrate phosphorylation by cyclin E-CDK2 and cyclin D2-CDK6 Homo sapiens ADP + RXL motif-containing phosphopeptide
-
 ?

Synonyms

Synonyms Comment Organism
CDK2
-
 Homo sapiens
cdk6
-
 Homo sapiens
KSHV cyclin
-
 Human gammaherpesvirus 8

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
evolution primate herpesviruses, including the oncogenic Kaposi sarcoma herpesvirus, encode cyclinDhomologues. Viral cyclins have diverged from their cellular progenitor in that they elicit holoenzyme activity independent of activating phosphorylation by the CDK-activating kinase and resistant to inhibition by CDK inhibitors Human gammaherpesvirus 8
additional information amino acids in cluster A and B modulate substrate recognition and specificity. Substrate phosphorylation by CDK6 with K-cyclin from Kaposi sarcoma herpesvirus, K-cyclin expression in U2OS cells leads to fragmentation of actin stress fibers Homo sapiens
physiological function cyclin/cyclin-dependent kinase (CDK) complexes are critical regulators of cellular proliferation. A complex network of regulatory mechanisms has evolved to control their activity, including activating and inactivating phosphorylation of the catalytic CDK subunit and inhibition through specific regulatory proteins, molecular mechanism, overview Homo sapiens
physiological function primate herpesviruses, including the oncogenic Kaposi sarcoma herpesvirus, encode cyclinDhomologues. Viral cyclins have diverged from their cellular progenitor in that they elicit holoenzyme activity independent of activating phosphorylation by the CDK-activating kinase and resistant to inhibition by CDK inhibitors, molecular mechanism, overview Human gammaherpesvirus 8