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Literature summary for 2.7.11.22 extracted from

  • Shu, F.; Lv, S.; Qin, Y.; Ma, X.; Wang, X.; Peng, X.; Luo, Y.; Xu, B.E.; Sun, X.; Wu, J.
    Functional characterization of human PFTK1 as a cyclin-dependent kinase (2007), Proc. Natl. Acad. Sci. USA, 104, 9248-9253.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
cyclin D3 i. e. CCND3, enhances phosphorylation of tumor suppressor Rb Homo sapiens

Application

Application Comment Organism
additional information PFTK1 acts as a CDK that regulates cell cycle progression and cell proliferation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
293T cells transfected with PFTK1 or mutant K146A, overexpression of PFTK1 by retroviral infection of U2OS cells Homo sapiens
expressed in HEK-293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K146A mutation of the invariant lysine in subdomain II critical for ATP binding Homo sapiens
K146A mutant is unable to bind ATP Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information CDK inhibitor p16INK4a fails to interact with PFTK1; p16INK4a fails to interact with PFTK1 Homo sapiens
p21Cip1 inhibits phosphorylation of tumor suppressor Rb Homo sapiens
p21Clp1 forms a ternary complex with and is negatively regulated by p21Cip1, but not by its truncated fragment p21Cip1-D3 Homo sapiens
siRNA knocking down of PFTK1 expression in SH-SY5Y cells, causes cell cycle arrest at G1 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 SDS-PAGE Homo sapiens
50000
-
 immunoprecipitation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
SH-SY5Y cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + retinoblastoma protein
-
 Homo sapiens ADP + phosphorylated retinoblastoma protein
-
 ?
ATP + tumor suppressor Rb
-
 Homo sapiens ADP + phosphorylated tumor suppressor Rb
-
 ?
additional information autophosphorylation activity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
CDK
-
 Homo sapiens
PFTK1
-
 Homo sapiens
PFTK1/CCND3 complex
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
cyclin D3 PFTK1 specifically interacted with cyclin D3 Homo sapiens