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Literature summary for 2.7.11.21 extracted from

  • Lera, R.F.; Potts, G.K.; Suzuki, A.; Johnson, J.M.; Salmon, E.D.; Coon, J.J.; Burkard, M.E.
    Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis (2016), Nat. Chem. Biol., 12, 411-418.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.21

Protein Variants

Protein Variants Comment Organism
additional information mutant Plk1as is resistant to inhibition by BI-2536 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
BI-2536 inhibits the wild-type enzyme but not mutant Plk1as Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
centromere
-
 Homo sapiens
-
-
kinetochore
-
 Homo sapiens 776
-
nucleus Plk1 localizes to several mitotic structures, including centrosomes, chromosome arms, kinetochores and the anaphase spindle midzone Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + BubR1 Homo sapiens
-
 ADP + phosphorylated BubR1
-
 ?
ATP + CENP-Q Homo sapiens
-
 ADP + phosphorylated CENP-Q
-
 ?
ATP + CLASP Homo sapiens
-
 ADP + phosphorylated CLASP
-
 ?
ATP + PBIP1 Homo sapiens
-
 ADP + phosphorylated PBIP1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NYY3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + BubR1
-
 Homo sapiens ADP + phosphorylated BubR1
-
 ?
ATP + CENP-Q
-
 Homo sapiens ADP + phosphorylated CENP-Q
-
 ?
ATP + CLASP
-
 Homo sapiens ADP + phosphorylated CLASP
-
 ?
ATP + PBIP1
-
 Homo sapiens ADP + phosphorylated PBIP1
-
 ?

Synonyms

Synonyms Comment Organism
Plk1
-
 Homo sapiens
Polo-like kinase 1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function polo-like kinase 1 (Plk1) is a core regulator of mitosis required for centrosome maturation, bipolar spindle formation, chromosome arm resolution, chromosome alignment and segregation, and cytokinesis. Protein kinase signaling along the kinetochore-centromere axis is crucial to assure mitotic fidelity. Deep within the centromere, Plk1 operates to assure proper chromosome alignment and segregation. Plk1 is recruited to the outer kinetochore by Bub1, NudC, and BubR1, where it phosphorylates BubR1 and CLASP to stabilize kinetochore-microtubule attachments, promoting chromosome alignment, and Kif2b to correct microtubule attachment errors, facilitating accurate chromosome segregation. At the inner kinetochore, Plk1's role in chromosome alignment and segregation also requires recruitment by and phosphorylation of CENP-U/50 (also called PBIP) and CENP-Q. Plk1 operates in pools within the kinetochore and the bulk pool is at the inner kinetochore/centromere, and it operates at chromatin and in the inner centromere in a manner that is distinct and separable from Plk1’s role in stabilizing microtubule attachments at the outer kinetochore Homo sapiens