Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.21 extracted from

  • Holland, A.J.; Lan, W.; Niessen, S.; Hoover, H.; Cleveland, D.W.
    Polo-like kinase 4 kinase activity limits centrosome overduplication by autoregulating its own stability (2010), J. Cell Biol., 188, 191-198.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.21

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and kinase-inactive (D154A) mutant enzyme are stably expressed in a human cell line (DLD-1) in which accurate chromosome segregation maintains a pseudodiploid karyotype Mus musculus

Protein Variants

Protein Variants Comment Organism
D154A kinase-inactive mutant enzyme Mus musculus

General Stability

General Stability Organism
kinase-active Plk4 is inherently unstable and targeted for degradation Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q64702
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein self-phosphorylation at multiple sites is required for Plk4 instability, indicating a requirement for a threshold level of Plk4 kinase activity to promote its own destruction Mus musculus

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Synonyms

Synonyms Comment Organism
PLK4
-
 Mus musculus
polo-like kinase 4
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

General Information

General Information Comment Organism
physiological function polo-like kinase 4 plays a key role in initiating centriole duplication. Kinase-mediated, autoregulated instability of Plk4 self-limits Plk4 activity so as to prevent centrosome amplification Mus musculus