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Literature summary for 2.7.11.21 extracted from
- Sequestration of Polo kinase to microtubules by phosphopriming-independent binding to Map205 is relieved by phosphorylation at a CDK site in mitosis (2008), Genes Dev., 22, 2707-2720.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pDONR221lambda entry vector, the gene is recombined in different destination vectors for expression in fusion with GFP or protein A | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L408A | mutant for interaction studies with Map205 | Drosophila melanogaster |
| V396A | mutant for interaction studies with Map205 | Drosophila melanogaster |
| W395F | mutant for interaction studies with Map205 | Drosophila melanogaster |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Map205 | Map205 can stabilize Polo and inhibit its cellular activity in vivo | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| centromere | - |
Drosophila melanogaster | - |
- |
| centrosome | - |
Drosophila melanogaster | 5813 | - |
| chromosome | - |
Drosophila melanogaster | 5694 | - |
| microtubule | - |
Drosophila melanogaster | 5874 | - |
| spindle | - |
Drosophila melanogaster | 5819 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + protein | Drosophila melanogaster | - |
ADP + phosphoprotein | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protein A-affinity purification is performed | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Drosophila melanogaster | - |
| larva | - |
Drosophila melanogaster | - |
| neuroblast | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + protein | - |
Drosophila melanogaster | ADP + phosphoprotein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLK | - |
Drosophila melanogaster |
| Polo kinase | - |
Drosophila melanogaster |
| polo-like kinase | - |
Drosophila melanogaster |
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Release 2023.1 (January 2023)
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