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Literature summary for 2.7.11.21 extracted from

  • Burkard, M.E.; Randall, C.L.; Larochelle, S.; Zhang, C.; Shokat, K.M.; Fisher, R.P.; Jallepalli, P.V.
    Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells (2007), Proc. Natl. Acad. Sci. USA, 104, 4383-4388.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
additional information late mitotic function of Plk1, kinase activity of Plk1 is needed for an early event in cytokinesis, acts upstream of RhoA, Plk1 activity is required for the equatorial localization of RhoA and its effectors during anaphase Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
transduction of PLK1flox/delta cells with retroviruses expressing either wild-type Plk1 or the equivalent double mutant C67V/L130G, as EGFP fusions Homo sapiens

Protein Variants

Protein Variants Comment Organism
C67V/L130G localizes to centrosomes and kinetochores in mitotic cells like the wild-type, is ca. 12fold less active than wild-type in the presence of ATP, both 1-NM-PP1 and 3-MB-PP1 inhibit rapidly, selectively, and reversibly its growth in a dose-dependent manner. Acute treatment during anaphase prevents recruitment of both Plk1 itself and the Rho guanine nucleotide exchange factor Ect2 to the central spindle, abolishes RhoA GTPase localization to the equatorial cortex, and suppresses cleavage furrow formation and cell division. 3-MB-PP1 elicits a more penetrant phenotype that is virtually identical to PLK1 deletion Homo sapiens
additional information homozygous deletion of PLK1 fully abrogates its function in vivo, PLK1delta/delta cells have monopolar or disorganized bipolar spindles with adjacent and immature centrosomes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information 1-NM-PP1 and 3-MB-PP1 have little effect on wild-type Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
centrosome
-
 Homo sapiens 5813
-
kinetochore
-
 Homo sapiens 776
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P53350
-
-

Source Tissue

Source Tissue Comment Organism Textmining
retinal pigment epithelium
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein
-
 Homo sapiens ADP + phosphorylated casein
-
 ?

Synonyms

Synonyms Comment Organism
Plk1
-
 Homo sapiens
Polo-like kinase 1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens