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Literature summary for 2.7.11.21 extracted from

  • Hansen, D.V.; Tung, J.J.; Jackson, P.K.
    CaMKII and Polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit (2006), Proc. Natl. Acad. Sci. USA, 103, 608-613.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.21

Application

Application Comment Organism
additional information is an essential factor for Ca2+-induced meiotic exit Xenopus laevis

Inhibitors

Inhibitors Comment Organism Structure
additional information only simultaneous mutation of both motifs 192RSST195 and 333RLST336 of Emi2 abrogates polo box domain binding of Plx1 Xenopus laevis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Emi2 only associates with Plx1 after Ca2+ addition, Ca2+-induced Emi2-Plx1 association in cytostatic factor extract relies mostly on the 192RSST motif, whereas the 333RLST motif contributes only slightly to Plx1 binding Xenopus laevis
Mg2+
-
 Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Emi2 Xenopus laevis
-
 ADP + phosphorylated Emi2
-
 ?
ATP + Emi2 Xenopus laevis CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit, in response to increased free Ca2+ levels CaMKII acts as a priming kinase mediating the interaction between Emi2 and Plx1 polo box domain via phosphorylation at a specific motif ADP + phosphorylated Emi2
-
 ?
additional information Xenopus laevis the enzyme is essential for Ca2+-induced meiotic exit of fertilized eggs after arrest in metaphase II before fertilization ?
-
 ?

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Emi2
-
 Xenopus laevis ADP + phosphorylated Emi2
-
 ?
ATP + Emi2 CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit, in response to increased free Ca2+ levels CaMKII acts as a priming kinase mediating the interaction between Emi2 and Plx1 polo box domain via phosphorylation at a specific motif Xenopus laevis ADP + phosphorylated Emi2
-
 ?
ATP + Emi2 Emi2, i.e. XErp1, is a cytostatic factor Xenopus laevis ADP + phosphorylated Emi2
-
 ?
ATP + Emi2 CaMKII specifically directs Plx1 activity toward Emi2 by functioning as a priming kinase, creating a phosphoepitope on Emi2 that recruits the polo box domain of Plx1, allowing subsequent phosphorylation of Emi2’s degron and its recognition by SCFbetaTrCP for ubiquitination and destruction Xenopus laevis ADP + phosphorylated Emi2
-
 ?
additional information the enzyme is essential for Ca2+-induced meiotic exit of fertilized eggs after arrest in metaphase II before fertilization Xenopus laevis ?
-
 ?

Synonyms

Synonyms Comment Organism
Plk1
-
 Xenopus laevis
Plx1
-
 Xenopus laevis
Polo-like kinase 1
-
 Xenopus laevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Xenopus laevis
30
-
 assay at Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Xenopus laevis
7.4
-
 assay at Xenopus laevis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Xenopus laevis