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Literature summary for 2.7.11.21 extracted from
- Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate (2003), J. Biol. Chem., 278, 25277-25280.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of HA-tagged Plk in HeLa cells, co-expression of His-tagged Plk with wild-type and mutant Myt1 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Myt1 | Homo sapiens | phosphorylation of Myt1 during M phase | ADP + phosphorylated Myt1 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant HA-tagged Plk from HeLa cells by immunoprecipitation | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | the Plk consensus sequence is D/E-X-S/T-hydrophobic amino acid-X-D/E with X being any amino acid | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Myt1 | phosphorylation of Myt1 during M phase | Homo sapiens | ADP + phosphorylated Myt1 | - |
? | |
| ATP + Myt1 | i.e. membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, no activity with a Myt1 mutant in which the 4 C-terminal phosphorylation sites are mutated to alanine | Homo sapiens | ADP + phosphorylated Myt1 | - |
? | |
| additional information | determination of enzyme consensus sequence | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLK | - |
Homo sapiens |
| polo-like kinase | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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