Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged enzyme in HeLa cells and LLCPK cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme inhibition by expression of siRNA or inhibition of enzyme expression in HeLa cells reducing the the level of 3F3/2 phosphoepitope and inhibiting normal kinetochore association, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetochore | - |
Homo sapiens | 776 | - |
kinetochore | - |
Xenopus laevis | 776 | - |
additional information | localization of the enzyme is dependent on cell cycle stage | Homo sapiens | - |
- |
additional information | localization of the enzyme is dependent on cell cycle stage | Xenopus laevis | - |
- |
spindle | - |
Homo sapiens | 5819 | - |
spindle pole | - |
Homo sapiens | 922 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3F3/2 kinase | Homo sapiens | the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity | ADP + phosphorylated 3F3/2 kinase | - |
? | |
ATP + 3F3/2 kinase | Xenopus laevis | the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity | ADP + phosphorylated 3F3/2 kinase | - |
? | |
additional information | Xenopus laevis | polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores | ? | - |
? | |
additional information | Homo sapiens | polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores, the enzym is required for association of Cdc20 to kinetochores | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Xenopus laevis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | consensus sequence of Plk1 is D/E-X-S/T-hydrophobic amino acid-X-D/E, X can be any amino acid, with neighbouring phosphorylation sequence L-Q-S-V-L-E being phosphorylated at the serine residue | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Xenopus laevis | - |
CFPAC-1 cell | - |
Homo sapiens | - |
egg | - |
Xenopus laevis | - |
HeLa cell | - |
Homo sapiens | - |
LLC-PK cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3F3/2 kinase | the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity | Homo sapiens | ADP + phosphorylated 3F3/2 kinase | - |
? | |
ATP + 3F3/2 kinase | the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity | Xenopus laevis | ADP + phosphorylated 3F3/2 kinase | - |
? | |
ATP + 3F3/2 kinase | purified recombinant Plk1 or M phase cell extract, phosphoepitope mapping reveals that the 3F3/2 kinase phosphoepitope overlaps the enzyme consensus phosphorylation seqence, overview | Homo sapiens | ADP + phosphorylated 3F3/2 kinase | - |
? | |
ATP + 3F3/2 kinase | purified recombinant Plk1 or M phase cell extract, phosphoepitope mapping reveals that the 3F3/2 kinase phosphoepitope overlaps the enzyme consensus phosphorylation seqence, overview | Xenopus laevis | ADP + phosphorylated 3F3/2 kinase | - |
? | |
additional information | polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores | Xenopus laevis | ? | - |
? | |
additional information | polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores, the enzym is required for association of Cdc20 to kinetochores | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Plk1 | - |
Xenopus laevis |
Plx1 | - |
Xenopus laevis |
Polo-like kinase 1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Xenopus laevis |