Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Plk2 is activated in vivo near the G1- to S-phase transition of the cell cycle | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and K111R mutant enzyme in CHO cells, overexpression of HA-tagged wild-type and mutant Plk2 in U2OS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K111R | site-directed mutagenesis, kinase inactive mutant, overexpression blocks the centriole duplication and arrests the cells in S-phase | Homo sapiens |
additional information | overexpression of dominant negative Plk2 mtant results in abolished centriole duplication in fibroblasts and in U2OS2 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
centrosome | Plk2 interaction with the centrosome is independent on enzyme kinase activity | Homo sapiens | 5813 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme is involved in regulation of centriole duplication cycle by protein phosphorylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
KE-37 cell | leukemic cell line | Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-casein | high activity of wild-type enzyme | Homo sapiens | ADP + phosphorylated alpha-casein | - |
? | |
additional information | the enzyme is involved in regulation of centriole duplication cycle by protein phosphorylation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk2 | - |
Homo sapiens |
polo-like kinase-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |