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Literature summary for 2.7.11.21 extracted from

  • Warnke, S.; Kemmler, S.; Hames, R.S.; Tsai, H.L.; Hoffmann-Rohrer, U.; Fry, A.M.; Hoffmann, I.
    Polo-like kinase-2 is required for centriole duplication in mammalian cells (2004), Curr. Biol., 14, 1200-1207.
    View publication on PubMed
Search for more literature for 2.7.11.21

Activating Compound

Activating Compound Comment Organism Structure
additional information Plk2 is activated in vivo near the G1- to S-phase transition of the cell cycle Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and K111R mutant enzyme in CHO cells, overexpression of HA-tagged wild-type and mutant Plk2 in U2OS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K111R site-directed mutagenesis, kinase inactive mutant, overexpression blocks the centriole duplication and arrests the cells in S-phase Homo sapiens
additional information overexpression of dominant negative Plk2 mtant results in abolished centriole duplication in fibroblasts and in U2OS2 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
centrosome Plk2 interaction with the centrosome is independent on enzyme kinase activity Homo sapiens 5813
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens
Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is involved in regulation of centriole duplication cycle by protein phosphorylation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
KE-37 cell leukemic cell line Homo sapiens
-
U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-casein high activity of wild-type enzyme Homo sapiens ADP + phosphorylated alpha-casein
-
 ?
additional information the enzyme is involved in regulation of centriole duplication cycle by protein phosphorylation Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Plk2
-
 Homo sapiens
polo-like kinase-2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens