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Literature summary for 2.7.11.20 extracted from

  • Wang, X.; Xie, J.; da Mota, S.R.; Moore, C.E.; Proud, C.G.
    Regulated stability of eukaryotic elongation factor 2 kinase requires intrinsic but not ongoing activity (2015), Biochem. J., 467, 321-331.
    View publication on PubMed
Search for more literature for 2.7.11.20

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on Homo sapiens

Application

Application Comment Organism
medicine the enzyme a possible target for therapeutic intervention in solid tumours Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ a Ca2+/calmodulin-dependent protein kinase Homo sapiens
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [elongation factor 2] Homo sapiens
-
 ADP + [elongation factor 2] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00418
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation site is Thr348 in eEF2K Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2] phosphate
-
 ?
additional information the enzyme performs autophosphorylation on residue Thr348 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
eEF2K
-
 Homo sapiens
eukaryotic elongation factor 2 kinase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction kinase-dead and other activity-deficient mutants of eEF2K are stabilized, as is a mutant lacking a critical autophosphorylation site (Thr348 in eEF2K), which is thought to be required for eEF2K and other alpha-kinases to achieve their active conformations. In contrast, application of small-molecule eEF2K inhibitors does not stabilize the protein. Achieving an active conformation, rather than eEF2K activity per se, is required for its susceptibility to degradation Homo sapiens
metabolism eEF2K is degraded by a proteasome-dependent pathway in response to genotoxic stress requiring a phosphodegron that includes an autophosphorylation site. The enzyme is also degraded under stress like including acidosis or treatment of cells with 2-deoxyglucose not requiring a phosphodegron Homo sapiens
physiological function eukaryotic elongation factor 2 kinase (eEF2K) is an atypical protein kinase which negatively regulates protein synthesis, is activated under stress conditions and plays a role in cytoprotection, e.g. in cancer cells Homo sapiens