Protein Variants | Comment | Organism |
---|---|---|
additional information | PDH kinase 1 knockout with siRNA, siRNA transfection into 832/13 clonal beta-cells, transfectant metabolite profiling compared to control cells, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial matrix | - |
Rattus norvegicus | 5759 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (acetyl-transferring)] | Rattus norvegicus | - |
ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
INS-1 832/13 cell | - |
Rattus norvegicus | - |
pancreatic beta cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (acetyl-transferring)] | - |
Rattus norvegicus | ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH kinase 1 | - |
Rattus norvegicus |
pyruvate dehydrogenase kinase 1 | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of PDH kinase 1 does not affect basal insulin secretion but, remarkably, insulin secretion in response to 16.7 mM glucose increases more vigorously than in negative control cells. The metabolite profile of cells with PDH kinase 1 knockdown is significantly different from that of the control cells, expecially the tricarboxylic acid cycle intermediates malate, fumarate and 2-oxoglutarate, overview | Rattus norvegicus |
metabolism | metabolic profiling and glucose-stimulated insulin secretion in pancreatic beta cells with and without knockdown of the enzyme, overview | Rattus norvegicus |