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Literature summary for 2.7.11.2 extracted from

  • Hiromasa, Y.; Hu, L.; Roche, T.E.
    Ligand-induced effects on pyruvate dehydrogenase kinase isoform 2 (2006), J. Biol. Chem., 281, 12568-12579.
    View publication on PubMed
Search for more literature for 2.7.11.2

Protein Variants

Protein Variants Comment Organism
W383F site-directed mutagenesis of isozyme PDHK2, the mutant shows unaltered catalytic activity compared to the wild-type isozyme PDHK2, but altered ligand binding and higher sensitivity to inhibition by pyruvate and ADP Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP binding kinetics,ATP or ADP plus pyruvate at low concentration of about 0.1 mM cause PDHK2 dimer to associate to a tetramer. These changes make major contributions to synergistic inhibition of PDHK2 activity by ADP and pyruvate, overview Homo sapiens
Dichloroacetate binding kinetics Homo sapiens
pyruvate binding kinetics,ATP or ADP plus pyruvate at low concentration of about 0.1 mM cause PDHK2 dimer to associate to a tetramer. These changes make major contributions to synergistic inhibition of PDHK2 activity by ADP and pyruvate, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PDHK2 is required for binding to the inner lipoyl domain L2 of the dihydrolipoyl acetyltransferase of the pyruvate dehydrogenase complex ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozyme PDHK2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PDHK2 is required for binding to the inner lipoyl domain L2 of the dihydrolipoyl acetyltransferase of the pyruvate dehydrogenase complex Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer or tetramer effects of ligand binding on distal structure of PDHK2, analytical ultracentrifugation, structure, overview Homo sapiens

Synonyms

Synonyms Comment Organism
PDHK
-
 Homo sapiens
pyruvate dehydrogenase kinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP binding kinetics, ATP or ADP plus pyruvate at low concentration of about 0.1 mM cause PDHK2 dimer to associate to a tetramer. These changes make major contributions to synergistic inhibition of PDHK2 activity by ADP and pyruvate, overview Homo sapiens