Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.2 extracted from

  • Thelen, J.J.; Miernyk, J.A.; Randall, D.D.
    Pyruvate dehydrogenase kinase from Arabidopsis thaliana: a protein histidine kinase that phosphorylates serine residues (2000), Biochem. J., 349, 195-201.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.2

Cloned(Commentary)

Cloned (Comment) Organism
functional expression as maltose-binding protein fusion protein in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
H121A site-directed mutagenesis, 50% decreased trans- and autophosphorylation activity Arabidopsis thaliana
H121Q site-directed mutagenesis, 50% decreased trans- and autophosphorylation activity Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arabidopsis thaliana 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, recombinant enzyme with maltose-binding protein cleaved off, SDS-PAGE Arabidopsis thaliana
86000
-
 approximately, recombinant enzyme with maltose-binding protein cleaved off, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] Arabidopsis thaliana the enzyme is the primary regulator of flux through the mitochondrial pyruvate dehydrogenase complex ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SBJ1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli as a fusion protein with the maltose-binding protein, to near homogeneity Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate mechanism Arabidopsis thaliana
a
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate His121 is involved in the catalytic reaction Arabidopsis thaliana
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] substrate is kinase-depleted pyruvate dehydrogenase complex from Zea mays Arabidopsis thaliana ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
ATP + [pyruvate dehydrogenase (lipoamide)] the enzyme is the primary regulator of flux through the mitochondrial pyruvate dehydrogenase complex Arabidopsis thaliana ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
additional information performs pH-dependent autophosphorylation on serine residues Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, recombinant enzyme with maltose-binding protein cleaved off, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 autophosphorylation Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Arabidopsis thaliana