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Literature summary for 2.7.11.2 extracted from
- Molecular cloning, functional expression, and characterization of pyruvate dehydrogenase kinase from anaerobic muscle of the parasitic nematode Ascaris suum (1998), Arch. Biochem. Biophys., 353, 181-189.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | kinase bound to transacetylase core | Ascaris suum |  |
| acetyl-CoA | synergism with NADH | Ascaris suum | |
| dihydrolipoyl transacetylase | pyruvate dehydrogenase-complex transacetylase core | Ascaris suum | |
| NADH | kinase bound to transacetylase core | Ascaris suum | |
| NADH | synergism with acetyl-CoA | Ascaris suum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis | Ascaris suum |
| functional expression in Escherichia coli strain BL21(DE3) as His-tagged protein | Ascaris suum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | enzyme contains a 18-amino acid mitochondrial import signal sequence | Ascaris suum | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Ascaris suum | |
| Mg2+ | actual substrate: MgATP2- | Ascaris suum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
about, recombinant enzyme, gel filtration | Ascaris suum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [pyruvate dehydrogenase (lipoamide)] | Ascaris suum | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | O02623 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli as His-tagged protein to homogeneity | Ascaris suum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | adult | Ascaris suum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ascaris suum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Ascaris suum | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
| ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Ascaris suum | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
| additional information | no autophosphorylation | Ascaris suum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000-43470, mature protein, SDS-PAGE and DNA sequence determination | Ascaris suum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ascaris suum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Ascaris suum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Ascaris suum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ascaris suum | mature protein | - |
5.9 |
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