Cloned (Comment) | Organism |
---|---|
expressed as a N-terminal GST fusion, expressed in B834 (DE3)pLyS cells at 18°C | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
indirubin | phosphorylase kinase ATP-binding site inhibitor | Oryctolagus cuniculus | |
indirubin-3'-oxime | phosphorylase kinase ATP-binding site inhibitor | Oryctolagus cuniculus | |
KT5720 | phosphorylase kinase ATP-binding site inhibitor | Oryctolagus cuniculus | |
staurosporine | phosphorylase kinase ATP-binding site inhibitor | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + glycogen phosphorylase b | - |
Oryctolagus cuniculus | 2 ADP + glycogen phosphorylase a | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000037 | - |
KT5720 | 30°C, pH 8.2 | Oryctolagus cuniculus | |
0.0000184 | - |
staurosporine | 30°C, pH 8.2 | Oryctolagus cuniculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000037 | - |
30°C, pH 8.2 | Oryctolagus cuniculus | staurosporine | |
0.0000184 | - |
30°C, pH 8.2 | Oryctolagus cuniculus | KT5720 | |
0.000144 | - |
30°C, pH 8.2 | Oryctolagus cuniculus | indirubin-3'-oxime | |
0.05 | - |
30°C, pH 8.2 | Oryctolagus cuniculus | indirubin |