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Literature summary for 2.7.11.19 extracted from

  • Andreeva, I.E.; Livanova, N.B.; Eronina, T.B.; Silonova, G.V.; Poglazov, B.F.
    Phosphorylase kinase from chicken skeletal muscle. Quaternary structure, regulatory properties and partial proteolysis (1986), Eur. J. Biochem., 158, 99-106.
    View publication on PubMed
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
Catalytic subunit of cAMP-dependent protein kinase activation of nonactivated enzyme Gallus gallus
chymotrypsin proteolytic activation of nonactivated enzyme Gallus gallus
additional information the nonactivated enzyme is activated either by limited proteolysis Gallus gallus
Trypsin proteolytic activation of nonactivated enzyme Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002 0.0025 phosphorylase b in the presence of glycogen Gallus gallus
0.019 0.02 phosphorylase b
-
 Gallus gallus
0.2
-
 ATP
-
 Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Gallus gallus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Gallus gallus
phosphate requirement, phosphate containing enzyme Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1320000
-
 gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.4
-
 hind limb Gallus gallus
19.6
-
 brest Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nonactivated phosphorylase kinase
-
 Gallus gallus ADP + activated phosphorylase kinase
-
 ?
ATP + phosphorylase b cosubstrate: Mg-ATP complex Gallus gallus ADP + phosphorylase a
-
 ?
ATP + phosphorylase b rabbit skeletal muscle Gallus gallus ADP + phosphorylase a
-
 ?

Subunits

Subunits Comment Organism
More gamma' subunit is not identical with rabbit skeletal muscle gamma subunit Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 0.3-0.5 Gallus gallus
8.5
-
-
 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8 progressive increase of activity, about half-maximal activity at pH 7.4 Gallus gallus