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Literature summary for 2.7.11.19 extracted from
- Phosphorylase kinase from chicken gizzard. Partial purification and characterization (1985), Eur. J. Biochem., 151, 467-473.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no autoactivation | Gallus gallus | |
| additional information | the nonactivated enzyme is activated either by limited proteolysis | Gallus gallus | |
| Trypsin | proteolytic activation of nonactivated enzyme | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | - |
Gallus gallus |  |
| phosphorylase b | high concentration | Gallus gallus | |
| quercetin | ATP does not reverse | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Gallus gallus | |
| 0.45 | - |
ATP | - |
Gallus gallus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement | Gallus gallus | |
| Mg2+ | requirement | Gallus gallus | |
| Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Gallus gallus | |
| phosphate | requirement, phosphate containing enzyme | Gallus gallus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to near homogeneity | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gizzard smooth muscle | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.53 | - |
- |
Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Gallus gallus | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | rabbit skeletal muscle | Gallus gallus | ADP + phosphorylase a | - |
? | |
| additional information | troponin (whole complex) and histone IIAS are no substrates | Gallus gallus | ? | - |
? | |
| additional information | phosvitin is no substrate | Gallus gallus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH 6.8/8.2 activity ratios: 0.58 | Gallus gallus |
| 9.3 | - |
- |
Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 9.3 | progressive increase of activity from 10% to 100% of maximal activity, with half-maximal activity at pH 7 | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | additional activation | Gallus gallus | |
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