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Literature summary for 2.7.11.19 extracted from
- Physiochemical properties of rabbit skeletal muscle phosphorylase kinase (1973), Biochemistry, 12, 567-573.
General Stability
| General Stability | Organism |
|---|---|
| isoelectric focusing inactivates | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTNB | only gradual loss of activity after more than 10 min, pH-dependent | Oryctolagus cuniculus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Oryctolagus cuniculus | |
| Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Oryctolagus cuniculus | |
| phosphate | requirement, phosphate containing enzyme | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Oryctolagus cuniculus |
| additional information | - |
enzyme aggregates to high polymeric forms which arise as artifacts during isolation procedure due to sensitivity to high hydrostatic pressure, e.g. during sucrose density gradient centrifugation at very high angular velocities | Oryctolagus cuniculus |
| 1330000 | - |
analytical ultracentrifugation | Oryctolagus cuniculus |
| 1330000 | - |
nonactivated enzyme | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Oryctolagus cuniculus | ADP + phosphorylase a | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.77 (nonactivated rabbit enzyme) | Oryctolagus cuniculus |
| additional information | - |
nonactivated kinase activity ratios at pH 6.8/8.2: between 0.01-0.05, activated kinase activity ratio: about 0.6 | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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