Cloned (Comment) | Organism |
---|---|
gene mylk5, expression analysis | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an antisense morpholino oligonucleotide is designed to induce mis-splicing of exon 9 of im:7148400 and early termination of the transcript before its kinase domain | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ML-7 | - |
Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | F1QPV1 | - |
- |
Danio rerio TL | F1QPV1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Danio rerio | - |
keratocyte | from 2 d or 4 d postfertilization (dpf) embryos, im:7148400 is the only MLCK isozyme in keratocytes | Danio rerio | - |
Synonyms | Comment | Organism |
---|---|---|
im:7148400 | - |
Danio rerio |
MLCK | - |
Danio rerio |
mylk5 | - |
Danio rerio |
myosin light chain kinase | - |
Danio rerio |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Danio rerio |
General Information | Comment | Organism |
---|---|---|
malfunction | decreasing MLCK activity in 4-dpf cells results in longer edge lifetimes and wider edges. Enzyme inhibition causes no change in the lateral propagation rate, but an increase in edge lifetime, which suggests that MLCK activity regulates edge size by controlling edge lifetime | Danio rerio |
physiological function | keratocytes from 2 d postfertilization (dpf) embryos resemble canonical fanshaped keratocytes, but keratocytes from 4 dpf embryos often form multiple protrusions despite unchanged membrane tension. 4-dpf Cells have multiple protrusions because the protrusions are intrinsically small. The multiple-protrusion phenotype is primarily due to increased myosin light chain kinase (MLCK) expression. MLCK activity influences cell polarity by increasing myosin accumulation in lamellipodia, which locally decreases protrusion lifetime, limiting lamellipodial size and allowing for multiple protrusions to coexist within the context of membrane tension limiting protrusion globally, while Rho kinase regulates myosin accumulation at the cell rear and does not determine protrusion size. MLCK-specific mechanism for controlling cell polarity via regulation of myosin activity in protrusions, overview. Enzyme inhibition causes no change in the lateral propagation rate, but an increase in edge lifetime, which suggests that MLCK activity regulates edge size by controlling edge lifetime | Danio rerio |