Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Gallus gallus | |
methylcellulose | enhances the rate of MLCK-induced phosphorylation of smooth muscle myosin and MLCK-induced mechanical activation of smooth muscle myosin to move actin filaments | Gallus gallus | |
additional information | kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system, overview. Actin has no activating effect on the enzyme activity | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the kinase activity of biotinylated-MLCK is comparable to MLCK without biotinylation using the regulatory light chain as the substrate. Biotinylated-MLCK and biotinylated-MLCK with attached streptavidin tag are able to fully phosphorylate smooth muscle myosin regulatory light chain | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system, kinetic modelling, overview. The dissociation of MLCK from phosphorylated smooth muscle myosin is rate-limiting and that the rate of the phosphorylation step is faster than this dissociation rate. Association to smooth muscle myosin is much faster than to smooth muscle myosin. The probability of MLCK interacting with unphosphorylated versus smooth muscle myosin is 55-460 times greater | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Gallus gallus | |
Mg2+ | required | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | Gallus gallus | - |
ADP + [myosin light chain] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P11799 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gizzard | - |
Gallus gallus | - |
smooth muscle | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | - |
Gallus gallus | ADP + [myosin light chain] phosphate | - |
? | |
ATP + [myosin light chain] | smooth muscle myosin regulatory light chain phosphorylation at Ser19 | Gallus gallus | ADP + [myosin light chain] phosphate | - |
? | |
additional information | static substrate binding structure of streptavidin-tagged enzyme, overview | Gallus gallus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
myosin light chain kinase | - |
Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Gallus gallus |
General Information | Comment | Organism |
---|---|---|
physiological function | MLCK-induced phosphorylation of smooth muscle myosin and MLCK-induced mechanical activation of smooth muscle myosin to move actin filaments, likely by increasing the time the MLCK remains close to the surface-attached myosin | Gallus gallus |